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Int J Biol Sci 2005; 1(3):103-109. doi:10.7150/ijbs.1.103

Research Paper

SDS-PAGE Electrophoretic Property of Human Chorionic Gonadotropin (hCG) and its β-subunit

Lay-Harn Gam, Aishah Latiff

1 School of Pharmaceutical Sciences, University Sciences of Malaysia, 11800 Penang, Malaysia.
2 Doping Control Centre, University Sciences of Malaysia, 11800 Penang, Malaysia.

Abstract

The microheterogeneity property of hCG with regards to its sialic acid contents resulted in variable mobility of the glycoprotein in SDS-PAGE. The intact hCG molecule is composed of two dissimilar subunits, namely α- and β-subunits. The identification of hCG bands in SDS-PAGE was accomplished by the immunoblotting experiment, whereby the antibody directed toward the specific region of β-subunit of hCG was used. The data shows that the different mobility of intact hCG was attributed to the different degree of desialylation of the glycoprotein. Nevertheless, unlike the intact hCG, the mobility of its β-subunit was not affected by its variety sialic acid content. This characteristic of β-hCG is beneficial when semi-quantification of total hCG is required. Quantification of hCG using the HPLC-reversed phase C18 analytical column is not possible as the glycoprotein was eluted in multiple fractions at different retention times. The identification of denatured hCG (HPLC eluted fractions) was carried out by immunoblotting experiment whilst immunoassay technique failed to detect its presence in any fraction.

Keywords: Human Chorionic Gonadotropin, heterogeneity, SDS-PAGE, immunoblotting

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How to cite this article:
Gam LH, Latiff A. SDS-PAGE Electrophoretic Property of Human Chorionic Gonadotropin (hCG) and its β-subunit. Int J Biol Sci 2005; 1(3):103-109. doi:10.7150/ijbs.1.103. Available from http://www.ijbs.com/v01p0103.htm