Int J Biol Sci 2007; 3(1):57-63. doi:10.7150/ijbs.3.57 This issue Cite

Short Research Communication

Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines

Tomonori Kawano

Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, Japan

Citation:
Kawano T. Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines. Int J Biol Sci 2007; 3(1):57-63. doi:10.7150/ijbs.3.57. https://www.ijbs.com/v03p0057.htm
Other styles

File import instruction

Abstract

Objectives: Studies have proposed two opposing roles for copper-bound forms of prion protein (PrP) as an anti-oxidant supporting the neuronal functions and as a pro-oxidant leading to neurodegenerative process involving the generation of reactive oxygen species. The aim of this study is to test the hypothesis in which putative copper-binding peptides derived from PrP function as possible catalysts for monoamine-dependent conversion of hydrogen peroxide to superoxide in vitro.

Materials and methods: Four peptides corresponding to the copper (II)-binding motifs in PrP were synthesized and used for analysis of peptide-catalyzed generation of superoxide in the presence of Cu (II) and other factors naturally present in the neuronal tissues.

Results: Among the Cu-binding peptides tested, the amino acid sequence corresponding to the Cu-binding site in the helical region was shown to be the most active for superoxide generation in the presence of Cu(II), hydrogen peroxide and aromatic monoamines, known precursors or intermediates of neurotransmitters. Among monoamines tested, three compounds namely phenylethylamine, tyramine and benzylamine were shown to be good substrates for superoxide-generating reactions by the Cu-bound helical peptide.

Conclusions: Possible roles for these reactions in development of prion disease were suggested.

Keywords: aromatic monoamine, copper, prion, redox, superoxide


Citation styles

APA
Kawano, T. (2007). Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines. International Journal of Biological Sciences, 3(1), 57-63. https://doi.org/10.7150/ijbs.3.57.

ACS
Kawano, T. Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines. Int. J. Biol. Sci. 2007, 3 (1), 57-63. DOI: 10.7150/ijbs.3.57.

NLM
Kawano T. Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines. Int J Biol Sci 2007; 3(1):57-63. doi:10.7150/ijbs.3.57. https://www.ijbs.com/v03p0057.htm

CSE
Kawano T. 2007. Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines. Int J Biol Sci. 3(1):57-63.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Popup Image