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Int J Biol Sci 2008; 4(3):176-183. doi:10.7150/ijbs.4.176

Research Paper

CO-releasing molecules (CORM-2)-liberated CO attenuates leukocytes infiltration in the renal tissue of thermally injured mice

Bingwei Sun, Zhiwei Sun, Qin Jin, Xi Chen

Department of Burns and Plastic Surgery, Affiliated Hospital, Jiangsu University, Zhenjiang 212001, Jiangsu Province, PR China

Abstract

Objective: To determine whether the CO-releasing molecule -liberated CO attenuates infiltration of leukocytes in the renal tissue of thermally injured mice.

Materials and methods: Twenty-eight mice were assigned to four groups. Mice in sham group (n=7) were underwent sham thermal injury, whereas mice in burn group (n=7) received 15% total body surface area (TBSA) full-thickness thermal injury. Mice in burn+CORM-2 group (n=7) underwent thermal injury followed by immediate administration of CORM-2 (8mg/kg, i.v.), whereas mice in burn+iCORM-2 group (n=7) underwent thermal injury followed by administration of iCORM-2 (an inactive compound used as negative control). Histological alterations and granulocytes infiltration in kidney were assessed alongised PMN accumulation, activation of NF-ĸΒ, expressions of ICAM-1 and HO-1 expression in renal tissues.

Results: Treatment of thermally injured mice with CORM-2 significantly attenuated PMN accumulation and prevented activation of NF-ĸΒ in the kidney. This was accompanied by a decrease of the expression of ICAM-1 and an increase in HO-1 expression. In parallel, burn-induced granulocytes infiltration in renal tissue was markedly decreased by treatment with CORM-2.

Conclusions: CO delivered by CORM-2 attenuates leukocytes infiltration in the kidney of burned mice by interfering with NF-ĸΒ activation, protein expression of ICAM-1 and therefore suppressing endothelial cells pro-adhesive phenotype.

Keywords: kidney, leukocyte infiltration, carbon monoxide, thermal injury

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How to cite this article:
Sun B, Sun Z, Jin Q, Chen X. CO-releasing molecules (CORM-2)-liberated CO attenuates leukocytes infiltration in the renal tissue of thermally injured mice. Int J Biol Sci 2008; 4(3):176-183. doi:10.7150/ijbs.4.176. Available from http://www.ijbs.com/v04p0176.htm