Int J Biol Sci 2011; 7(3):347-363. doi:10.7150/ijbs.7.347
MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3
1. Department of Radiation Medicine, Second Military Medical University, Xiangyin Road, Shanghai 200433, PR China;
Dysregulation of certain microRNAs (miRNAs) in cancer can promote tumorigenesis, metastasis and invasion. However, the functions and targets of only a few mammalian miRNAs are known. In particular, the miRNAs that participates in radiation induced carcinogenesis and the miRNAs that target the tumor suppressor gene Big-h3 remain undefined. Here in this study, using a radiation induced thymic lymphoma model in BALB/c mice, we found that the tumor suppressor gene Big-h3 is down-regulated and miR-21 is up-regulated in radiation induced thymic lymphoma tissue samples. We also found inverse correlations between Big-h3 protein and miR-21 expression level among different tissue samples. Furthermore, our data indicated that miR-21 could directly target Big-h3 in a 3′UTR dependent manner. Finally, we found that miR-21 could be induced by TGFβ, and miR-21 has both positive and negative effects in regulating TGFβ signaling. We conclude that miR-21 participates in radiation induced carcinogenesis and it regulates TGFβ signaling.
Keywords: Radiation-induced carcinogenesis, Radiation induced thymic lymphoma, MicroRNA, miR-21, Big-h3, TGFβ.
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How to cite this article:
Liu C, Li B, Cheng Y, Lin J, Hao J, Zhang S, Mitchel REJ, Sun D, Ni J, Zhao L, Gao F, Cai J. MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. Int J Biol Sci 2011; 7(3):347-363. doi:10.7150/ijbs.7.347. Available from http://www.ijbs.com/v07p0347.htm