Int J Biol Sci
2011; 7(5):618-628.
doi:10.7150/ijbs.7.618 This issueCite
Research Paper
Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity
1. The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China 2. The State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China 3. Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China 4. Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, 710038 Xi'an, China * These authors contributed equally to this article.
✉ Corresponding author: Yingqi Zhang and Zhen Yan. Yingqi Zhang: The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics of The Fourth Military Medical University, 17 Changle West Road, 710032 Xi'an, P. R. China. Phone: +86-2984774775; Fax: +86-2983247213; E-mail: zhangyqhedu.cn. Zhen Yan: The State Key Laboratory of Cancer Biology, Department of Pharmacogenomics of The Fourth Military Medical University, 17 Changle West Road, 710032 Xi'an, P. R. China. Phone: +86-2984774769; Email: yanzhenedu.cnMore
Citation:
Li W, Song L, Wu S, Xue X, Zhang L, He L, Han W, Wang Q, Ling R, Zhang W, Yan Z, Zhang Y. Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity. Int J Biol Sci 2011; 7(5):618-628. doi:10.7150/ijbs.7.618. https://www.ijbs.com/v07p0618.htm
Thymosin alpha 1 (Tα1) has immunomodulatory and anti-tumor effects in patients and has been commercialized in worldwide. An innovative technique is therefore impending to achieve high-yield expression and purification of Tα1 to meet the increasing requirements for clinical applications. Tα1 can enhance T cells, dendritic cells and antibody responses, and also augment an anti-tumor immune response. In the current study, we developed a novel technique to produce Tα1 concatemer and investigated its capability in anti-tumor immunotherapy. We expressed the recombinant 2×Tα1 concatemer protein (Tα1② protein) in Escherichia coli. The purity of Tα1② was higher than 95% as assessed by HPLC analysis. In vitro, Tα1② could stimulate the proliferation of mouse splenic lymphocyte, and increase the apoptosis of tumor cell lines. In vivo, Tα1② significantly inhibited the tumor growth in B16 tumor-bearing mice. Compared with Tα1, the Tα1② is of more effective bioactivity than Tα1. The purified Tα1② is a promising substitute for synthetic Tα1 because of its potent anti-tumor effects. We concluded that the expression system for Tα1 concatemer was constructed successfully, which could serves as a highly efficient tool for the production of large quantities of the highly active protein.
Keywords: Thymosin alpha 1, concatemer, protein expression, purification, tumor suppression
Citation styles
APA
Li, W., Song, L., Wu, S., Xue, X., Zhang, L., He, L., Han, W., Wang, Q., Ling, R., Zhang, W., Yan, Z., Zhang, Y. (2011). Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity. International Journal of Biological Sciences, 7(5), 618-628. https://doi.org/10.7150/ijbs.7.618.
ACS
Li, W.; Song, L.; Wu, S.; Xue, X.; Zhang, L.; He, L.; Han, W.; Wang, Q.; Ling, R.; Zhang, W.; Yan, Z.; Zhang, Y. Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity. Int. J. Biol. Sci. 2011, 7 (5), 618-628. DOI: 10.7150/ijbs.7.618.
NLM
Li W, Song L, Wu S, Xue X, Zhang L, He L, Han W, Wang Q, Ling R, Zhang W, Yan Z, Zhang Y. Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity. Int J Biol Sci 2011; 7(5):618-628. doi:10.7150/ijbs.7.618. https://www.ijbs.com/v07p0618.htm
CSE
Li W, Song L, Wu S, Xue X, Zhang L, He L, Han W, Wang Q, Ling R, Zhang W, Yan Z, Zhang Y. 2011. Expression, Purification and Characterization of A Novel Soluble Human Thymosin Alpha1 Concatemer Exhibited A Stronger Stimulation on Mice Lymphocytes Proliferation and Higher Anti-tumor Activity. Int J Biol Sci. 7(5):618-628.