Int J Biol Sci 2011; 7(7):978-991. doi:10.7150/ijbs.7.978
PTPBR7 Binding Proteins in Myelinating Neurons of the Mouse Brain
1. Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 28, 6525 GA Nijmegen, The Netherlands
Mouse protein tyrosine phosphatase PTPBR7 is a receptor-like, transmembrane protein that is localized on the surface of neuronal cells. Its protein phosphatase activity is reduced upon multimerization, and PTPBR7-deficient mice display motor coordination defects. Extracellular molecules that may influence PTPBR7 activity, however, remain to be determined. We here show that the PTPBR7 extracellular domain binds to highly myelinated regions in mouse brain, in particular the white matter tracks in cerebellum. PTPBR7 deficiency does not alter this binding pattern, as witnessed by RAP in situ staining of Ptprr-/- mouse brain sections. Additional in situ and in vitro experiments also suggest that sugar moieties of heparan sulphate and chondroitin sulphate glycosaminoglycans are not critical for PTPBR7 binding. Candidate binding proteins were affinity-purified exploiting the PTPBR7 extracellular domain and identified by mass spectrometric means. Results support the suggested link between PTPRR isoforms and cerebellar calcium ion homeostasis, and suggest an additional role in the process of cell-cell adhesion.
Keywords: cerebellum, RAP in situ, extracellular domain, ligand protein, PTPRR, protein tyrosine phosphatase, mass spectrometry.
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How to cite this article:
Chesini IM, Debyser G, Croes H, ten Dam GB, Devreese B, Stoker AW, Hendriks WJAJ. PTPBR7 Binding Proteins in Myelinating Neurons of the Mouse Brain. Int J Biol Sci 2011; 7(7):978-991. doi:10.7150/ijbs.7.978. Available from http://www.ijbs.com/v07p0978.htm