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Int J Biol Sci 2011; 7(9):1334-1344. doi:10.7150/ijbs.7.1334

Review

Paratransgenic Control of Vector Borne Diseases

Ivy Hurwitz, Annabeth Fieck, Amber Read, Heidi Hillesland*, Nichole Klein, Angray Kang1, Ravi Durvasula

Center for Global Health, Department of Internal Medicine, University of New Mexico and New Mexico VA Health Care System, Albuquerque, New Mexico, USA.
1. Institute of Dentistry, Queen Mary, University of London, England.
* Present address: Department of Internal Medicine, University of Washington Medical Center, Seattle, Washington, USA.

Abstract

Conventional methodologies to control vector borne diseases with chemical pesticides are often associated with environmental toxicity, adverse effects on human health and the emergence of insect resistance. In the paratransgenic strategy, symbiotic or commensal microbes of host insects are transformed to express gene products that interfere with pathogen transmission. These genetically altered microbes are re-introduced back to the insect where expression of the engineered molecules decreases the host's ability to transmit the pathogen. We have successfully utilized this strategy to reduce carriage rates of Trypanosoma cruzi, the causative agent of Chagas disease, in the triatomine bug, Rhodnius prolixus, and are currently developing this methodology to control the transmission of Leishmania donovani by the sand fly Phlebotomus argentipes. Several effector molecules, including antimicrobial peptides and highly specific single chain antibodies, are currently being explored for their anti-parasite activities in these two systems. In preparation for eventual field use, we are actively engaged in risk assessment studies addressing the issue of horizontal gene transfer from the modified bacteria to environmental microbes.

Keywords: Paratransgenesis, Chagas disease, triatomine bugs, visceral leishmaniasis, sand flies, microbiology, risk assessment, horizontal gene transfer

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Hurwitz I, Fieck A, Read A, Hillesland H, Klein N, Kang A, Durvasula R. Paratransgenic Control of Vector Borne Diseases. Int J Biol Sci 2011; 7(9):1334-1344. doi:10.7150/ijbs.7.1334. Available from http://www.ijbs.com/v07p1334.htm