Int J Biol Sci 2012; 8(10):1408-1417. doi:10.7150/ijbs.4597
Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1
1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China;
2. Guangdong Province Engineering and technology R&D centers for pig breeding, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China.
Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.
Keywords: miR-145, IRS1, inhibit, dedifferentiated fat cells, adipogenesis.
Guo Y, Chen Y, Zhang Y, Zhang Y, Chen L, Mo D. Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1. Int J Biol Sci 2012; 8(10):1408-1417. doi:10.7150/ijbs.4597. Available from http://www.ijbs.com/v08p1408.htm