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Int J Biol Sci 2013; 9(3):289-294. doi:10.7150/ijbs.5928
Association between the C.1161G>A and C.1779C>G Genetic Variants of XRCC1 Gene and Hepatocellular Carcinoma Risk in Chinese Population
Department of Oncology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
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How to cite this article:
Deng X, Liang J, Jiang M, Zhou X, Liu H. Association between the C.1161G>A and C.1779C>G Genetic Variants of XRCC1 Gene and Hepatocellular Carcinoma Risk in Chinese Population. Int J Biol Sci 2013; 9(3):289-294. doi:10.7150/ijbs.5928. Available from http://www.ijbs.com/v09p0289.htm
The human X-ray repair complementing group 1 gene (XRCC1) is an important candidate gene influencing hepatocellular carcinoma (HCC) susceptibility. The objective of this study was to detect the association between c.1161G>A and c.1779C>G variants of XRCC1 gene and HCC risk. This study was conducted in Chinese population consisting of 623 HCC cases and 639 controls. These two genetic variants could be genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The association of XRCC1 gene variants with the risk of HCC was investigated under different genetic models. Our findings suggested that the genotypes/alleles from c.1161G>A and c.1779C>G genetic variants were statistically associated with HCC risk. As for the c.1161G>A, the AA genotype was statistically associated with the increased risk of HCC compared to GG wild genotype (OR = 2.36, 95% CI 1.63-3.40, P < 0.001). As for the c.1779C>G, the risk of HCC was significantly higher for GG genotype compared to CC wild genotype (OR = 2.17, 95% CI 1.51-3.12, P < 0.001). Furthermore, significant differences in the risk of HCC were also detected in other genetic models for these two variants. The allele-A of c.1161G>A and allele-G of c.1779C>G variants may contribute to the susceptibility of HCC (A versus G: OR = 1.48, 95% CI 1.26-1.75, P < 0.001 and G versus C: OR = 1.51, 95% CI 1.28-1.78, P < 0.001). Our data indicated that these two variants of XRCC1 gene were statistically associated with HCC risk in Chinese population.
Keywords: Hepatocellular carcinoma, XRCC1 gene, genetic variants, molecular markers, susceptibility, risk factors.
Hepatocellular carcinoma (HCC) is the fifth common cancer and the third leading cause of cancer-related death globally. It is a global health problem and a common liver malignancy in the world [1-3]. Several studies suggest that HCC shows great geographical variation, and more than 600,000 people die from HCC every year, and about > 75% of these HCC cases occur in the Asia-Pacific region [4,5]. China has a very high HCC incidence, with approximately 55% of annual new cases of HCC in the world [1,6,7]. Since the 1990s, HCC has been the second leading cause of cancer deaths in China . Up to date, the exact mechanism of HCC remains poorly understood. There were several studies suggested that the human X-ray repair complementing group 1 gene (XRCC1) was an important candidate gene which influencing HCC risk [9-21]. Many single nucleotide polymorphisms (SNPs) in XRCC1 gene, such as Arginine (Arg)194 Tryptophan (Trp), Arg280 Histidine (His) and Arg399 Glutanine (Gln), have been analyzed to evaluate the possible association between the genetic variants of XRCC1 gene and the risk of HCC [9-16]. Fortunately, several studies have proved that the genetic variants of XRCC1 gene were associated with HCC [9-16]. However, there were no similar studies on the association between c.1161G>A and c.1779C>G genetic variants and the risk of HCC. Therefore, the purpose of this study was to assess whether these two genetic variants influence the risk of HCC.
Materials and methods
A total of 623 HCC patients and 639 cancer-free controls were enrolled from Ruikang Hospital (Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, China) between January 2009 to December 2011 in this case-control study. All HCC patients and cancer-free controls were unrelated Han Chinese. HCC patients were diagnosed by doctors based on the standards established by Chinese Society of Liver Cancer (CSLC). Cancer-free controls were matched with HCC patients with regard to gender and age, excluding those with a history of HCC and other medical diseases. The clinical characteristics were collected in Table 1, including gender, age, alcohol drinking, tobacco smoking, hypertension, diabetes mellitus, family history of HCC, hepatitis B virus ( HBV) serological markers, and serum alpha-fetoprotein (a-FP) levels. This study was approved by the ethics committee of Ruikang Hospital (Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, China). Informed consent was obtained from all the subjects enrolled in the study.
XRCC1 variants genotyping
Genomic DNA was isolated from venous blood of all the subjects using the standard protocol . PCR primers were designed using Primer Premier 5.0 software. Primers, region, fragment size, and annealing temperature were summarized in Table 2. XRCC1 genetic variants were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and almost 10% of the samples showing genetic polymorphisms for XRCC1 gene were verified by DNA sequencing (ABI3730xl DNA Analyzer, Applied Biosystems, Foster City, CA) to ensure the concordance of the PCR-RFLP results. The PCR reactions were performed in a total volume of 20 µL solution containing 50ng template DNA, 1×buffer (Tris-HCl 100 mmol/L, pH 8.3; KCl 500 mmol/L), 0.25 µmol/L primers, 2.0 mmol/L MgCl2, 0.25 mmol/L dNTPs (Bioteke Corporation, Beijing, China), and 0.5U Taq DNA polymerase (Promega, Madison, WI, USA). The PCR conditions were as follows: initial denaturation at 94°C for 5 min, followed by 35 cycles at 94°C for 30 s, at the corresponding temperature (given in Table 2) for 30 s, at 72°C for 30 s, and a final extension at 72°C for 5 min. The PCR amplified products were digested with 5 units corresponding restriction enzyme (MBI Fermentas, St. Leon-Rot, Germany, Table 2) at 37°C for 10 h following the supplier's manual and electrophoresed on 2.5% agarose gel containing ethidium-bromide.
The chi-squared (χ2) test was performed to assess the Hardy-Weinberg equilibrium in genotype/allele frequencies, and compare the differences of the clinical characteristics between HCC patients and cancer-free controls. The odds ratios (ORs) with its 95% confidence intervals (95% CIs) of the association between allele/genotype frequencies and the risk of HCC were evaluated by multivariate logistic regression models. A P value < 0.05 was suggested as statistically significant. The statistical analyses were analyzed using the Statistical Package for Social Sciences software (SPSS, Windows version release 15.0; SPSS Inc.; Chicago, IL, USA).
Subjects and general characteristics
In total, 1262 subjects were recruited in this case-control study, including 623 HCC cases and 639 cancer-free controls. The clinical characteristics of subjects were performed in Table 1. There was no statistically significant difference between HCC patients and cancer-free controls in terms of age and gender (P = 0.3851 and P = 0.4423, respectively). Furthermore, no statistically significant differences were obtained in other clinical characteristics, such as alcohol drinking, tobacco smoking, hypertension, and diabetes mellitus between HCC cases and cancer-free controls (P = 0.3115, P = 0.6512, P = 0.8074 and P = 0.1363, respectively).
Identification and genotyping of XRCC1 variants
Two allelic variants (c.1161G>A and c.1779C>G) were found by PCR-RFLP and DNA sequencing methods in this study. Based on the reference sequences GenBank ID: NC_000019.9, NM_006297.2 and NP_006288.2, the sequence analysis suggested that c.1161G>A allelic variant was a synonymous mutation, which caused by G to A mutations in exon10 of XRCC1 gene [p. Leucine (Leu) 387Leu]. As for the c.1779C>G variant, sequence analysis proved that this variant was caused by C to G mutations in exon16 of XRCC1 gene, which leading to the Serine (Ser) to Arginine amino acid (Arg) replacement (p.Ser593Arg). The PCR amplified products of c.1161G>A variant was digested with MaeI restriction enzyme and divided into three genotypes, GG (213 bp), GA (213, 183 and 30 bp) and AA (183 and 30 bp, Table 2). As for the c.1779C>G variant, it was digested with AluI restriction enzyme and divided into three genotypes, CC (177 and 58 bp), CG (235, 177 and 58 bp) and GG (235 bp, Table 2). The genotype/allele frequencies of these two genetic variants in both HCC patients and cancer-free controls were performed in Table 3. As shown in Table 3, the genotype distributions of these two genetic variants in the subjects didn't significantly deviate from Hardy-Weinberg equilibrium (all P values > 0.05). Allele-G and allele-C were predominant alleles in the studied group for c.1161G>A and c.1779C>G variants, respectively. As for the c.1161G>A variant, the allele frequencies of HCC patients (G, 61.16%; A, 38.84%) were statistically significantly different from cancer-free controls (G, 70.03%; A, 29.97%; χ2 = 22.0390, P < 0.001). Genotype frequencies in HCC patients were not consistent with cancer-free controls, the differences being statistically significant (χ2 = 22.4559, P < 0.001). As for the c.1779C>G variant, the allele frequencies of HCC patients (C, 61.08%; G, 38.92%) were statistically significantly different from cancer-free controls (C, 70.34%; G, 29.66%, χ2 = 24.075, P < 0.001). The genotype distributions in HCC patients were statistically significantly different from cancer-free controls (χ2 = 23.4151, P < 0.001, Table 3).
XRCC1 variants and the risk of HCC
We found that the genotypes/alleles from the c.1161G>A and c.1779C>G variants were statistically associated with the risk of HCC (Table 4). As for the c.1161G>A variant, statistically significantly increased risk of HCC were detected in the homozygote comparison (AA versus (vs.) GG: OR = 2.36, 95% CI 1.63-3.40, χ2 = 21.63, P < 0.001), heterozygote comparison (GA vs. GG: OR = 1.34, 95% CI 1.06-1.70, χ2 = 5.79, P = 0.016), dominant model (AA/GA vs. GG: OR = 1.51, 95% CI 1.21-1.89, χ2 = 13.17, P < 0.001), recessive model (AA vs. GA/GG: OR = 2.04, 95% CI 1.44-2.88, χ2 = 16.66, P = < 0.001) and allele contrast (A vs. G: OR = 1.48, 95% CI 1.26-1.75, χ2 = 22.03, P < 0.001, Table 4). Similarly, as for the c.1779C>G variant, we also found that there were statistically significant differences in the homozygote comparison (GG vs. CC: OR = 2.17, 95% CI 1.51-3.12, χ2 = 18.07, P < 0.001), heterozygote comparison (CG vs. CC: OR = 1.55, 95% CI 1.22-1.96, χ2 = 12.98, P < 0.001), dominant model (GG/GC vs. CC: OR = 1.67, 95% CI 1.33-2.09, χ2 = 20.03, P < 0.001), recessive model (GG vs. GC/CC: OR = 1.75, 95% CI 1.24-2.46, χ2 = 10.44, P = 0.001) and allele contrast (G vs. C: OR = 1.51, 95% CI 1.28-1.78, χ2 = 24.07, P < 0.001, Table 4).
The clinical characteristics of hepatocellular carcinoma (HCC) cases and controls.
Primers and PCR-RFLP analysis for genotyping XRCC1 gene polymorphisms.
Note: SNPs, single nucleotide polymorphisms; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism.
The genotypic and allelic frequencies of XRCC1 polymorphisms in case and groups.
Association between hepatocellular carcinoma (HCC) risk and XRCC1 gene polymorphisms.
SNPs, single nucleotide polymorphisms; OR, odds ratio; CI, confidence interval; vs., versus.
Emerging evidence suggests that HCC is a common and polygenic malignant solid cancers resulting from complex interactions between environmental and genetic factors [23-27]. It represents a major and constantly rising health burden worldwide. Previous studies have approved that genetic factors have the key roles in the pathogenesis of HCC [28-32]. In recent years, the XRCC1 gene is regarded as an important candidate gene for HCC, and several association studies have been conducted to assess the role of Arg194Trp, Arg280His and Arg399Gln variants on the risk of HCC [9-16]. The findings from the previous association studies still remain conflicting rather than conclusive [9-16]. Kiran et al., indicated that Arg194Trp, Arg280His and Arg399Gln variants were significantly associated with the risk of hepatitis virus related HCC in Indian population . Pan et al., suggested that the Arg399Cln Arg/Gln indicated an increased risk of HCC, especially for patients above 50 years old or with drinking habits in Chinese population . However, Liu et al., demonstrated that the Arg399Gln is not statistically associated with altered HCC susceptibility according to the findings from meta-analysis method . However, up to date, the c.1161G>A and c.1779C>G genetic variants have not been analyzed in the risk of HCC. In this case-control study, we evaluated the association between these two SNPs and the risk of HCC in Chinese Han population. Results from this study, statistical significant differences were shown in allele and genotype frequencies between HCC patients and cancer-free controls for these two genetic variants (Table 3). As for the c.1161G>A variant, the risk of HCC was significantly higher for AA genotype compared to GG genotype and GA/GG carriers. As for the c.1779C>G variant, the GG genotype was significantly associated with the increased risk of HCC compared to CC genotype and CG/CC carriers. The allele-A of c.1161G>A and allele-G of c.1779C>G variants could contribute to the risk of HCC (Table 4), and these two alleles might be associated with a protection from HCC. These genetic variants might affect the function of XRCC1 protein in base excision DNA repair pathway, which is significantly associated with the risk of HCC. The results from the current study might provide more evidences to assess the role of XRCC1 gene in the development of HCC. Our date indicated that the c.1161G>A and c.1779C>G variants of XRCC1 gene were statistically associated with the increased risk of HCC in Chinese Han population.
In conclusion, to the best of our knowledge, this is the first investigation about the association between XRCC1 gene c.1161G>A and c.1779C>G genetic variants and HCC risk. Our findings supported that the XRCC1 genetic variants may be used as molecular markers for assessing the risk of HCC. Further studies will be needed to confirm these findings and explain the molecular mechanisms between XRCC1 genetic variants and HCC risk on larger different populations.
The study was financially supported by the International Cooperation Project, the Ministry of Science and Technology of the People's Republic of China (S2012ZR0128), and the Traditional Chinese Medicine Research Project, Guangxi Administration of Traditional Chinese Medicine (GZKZ-Z1103).
The authors have declared that no competing interest exists.
1. Parkin DM, Bray F, Ferlay J. et al. Global cancer statistics, 2002. CA Cancer J Clin. 2005;55(2):74-108
2. Llovet JM, Burroughs A, Bruix J. Hepatocellular carcinoma. Lancet. 2003;362(9399):1907-1917
3. Parikh S, Hyman D. Hepatocellular cancer: a guide for the internist. Am J Med. 2007;120(3):194-202
4. But DY, Lai CL, Yuen MF. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol. 2008;14(11):1652-1656
5. Yuen MF, Hou JL, Chutaputti A. Hepatocellular carcinoma in the Asia pacific region. J Gastroenterol Hepatol. 2009;24(3):346-353
6. Parkin DM. Global cancer statistics in the year 2000. Lancet Oncol. 2001;2(9):533-543
7. Schutte K, Bornschein J, Malfertheiner P. Hepatocellular carcinoma--epidemiological trends and risk factors. Dig Dis. 2009;27(2):80-92
8. Chen JG, Zhang SW, Chen WQ. [Analysis of liver cancer mortality in the national retrospective sampling survey of death causes in China, 2004 - 2005]. Zhonghua Yu Fang Yi Xue Za Zhi. 2010;44(5):383-389
9. Rossit AR, Cabral IR, Hackel C. et al. Polymorphisms in the DNA repair gene XRCC1 and susceptibility to alcoholic liver cirrhosis in older Southeastern Brazilians. Cancer Lett. 2002;180(2):173-182
10. Yu MW, Yang SY, Pan IJ. et al. Polymorphisms in XRCC1 and glutathione S-transferase genes and hepatitis B-related hepatocellular carcinoma. J Natl Cancer Inst. 2003;95(19):1485-1488
11. Kiran M, Chawla YK, Jain M. et al. Haplotypes of microsomal epoxide hydrolase and x-ray cross-complementing group 1 genes in Indian hepatocellular carcinoma patients. DNA Cell Biol. 2009;28(11):573-577
12. Kiran M, Saxena R, Chawla YK. et al. Polymorphism of DNA repair gene XRCC1 and hepatitis-related hepatocellular carcinoma risk in Indian population. Mol Cell Biochem. 2009;327(1-2):7-13
13. Pan HZ, Liang J, Yu Z. et al. Polymorphism of DNA repair gene XRCC1 and hepatocellular carcinoma risk in Chinese population. Asian Pac J Cancer Prev. 2011;12(11):2947-2950
14. Li QW, Lu CR, Ye M. et al. Evaluation of DNA repair gene XRCC1 polymorphism in prediction and prognosis of hepatocellular carcinoma risk. Asian Pac J Cancer Prev. 2012;13(1):191-194
15. Han X, Xing Q, Li Y. et al. Study on the DNA Repair Gene XRCC1 and XRCC3 Polymorphism in Prediction and Prognosis of Hepatocellular Carcinoma Risk. Hepatogastroenterology. 2012 epub
16. Long XD, Ma Y, Huang HD. et al. Polymorphism of XRCC1 and the frequency of mutation in codon 249 of the p53 gene in hepatocellular carcinoma among Guangxi population, China. Mol Carcinog. 2008;47(4):295-300
17. Liu F, Li B, Wei Y. et al. XRCC1 genetic polymorphism Arg399Gln and hepatocellular carcinoma risk: a meta-analysis. Liver Int. 2011;31(6):802-809
18. Borentain P, Gerolami V, Ananian P. et al. DNA-repair and carcinogen-metabolising enzymes genetic polymorphisms as an independent risk factor for hepatocellular carcinoma in Caucasian liver-transplanted patients. Eur J Cancer. 2007;43(17):2479-2486
19. Long XD, Ma Y, Wei YP. et al. The polymorphisms of GSTM1, GSTT1, HYL1*2, and XRCC1, and aflatoxin B1-related hepatocellular carcinoma in Guangxi population, China. Hepatol Res. 2006;36(1):48-55
20. Chen CC, Yang SY, Liu CJ. et al. Association of cytokine and DNA repair gene polymorphisms with hepatitis B-related hepatocellular carcinoma. Int J Epidemiol. 2005;34(6):1310-1318
21. Kirk GD, Turner PC, Gong Y. et al. Hepatocellular carcinoma and polymorphisms in carcinogen-metabolizing and DNA repair enzymes in a population with aflatoxin exposure and hepatitis B virus endemicity. Cancer Epidemiol Biomarkers Prev. 2005;14(2):373-379
22. Daly AK, Steen VM, Fairbrother KS. et al. CYP2D6 multiallelism. Methods Enzymol. 1996;272:199-210
23. Farazi PA, DePinho RA. Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer. 2006;6(9):674-687
24. El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology. 2007;132(7):2557-2576
25. Amarapurkar DN, Patel ND, Kamani PM. Impact of diabetes mellitus on outcome of HCC. Ann Hepatol. 2008;7(2):148-151
26. Marrero JA, Fontana RJ, Fu S. et al. Alcohol, tobacco and obesity are synergistic risk factors for hepatocellular carcinoma. J Hepatol. 2005;42(2):218-224
27. Yu L, Sun L, Jiang YF. et al. Interactions between CYP1A1 polymorphisms and cigarette smoking are associated with the risk of hepatocellular carcinoma: evidence from epidemiological studies. Mol Biol Rep. 2012;39(6):6641-6646
28. Nault JC, Zucman-Rossi J. Genetics of hepatobiliary carcinogenesis. Semin Liver Dis. 2011;31(2):173-187
29. Thorgeirsson SS, Grisham JW. Molecular pathogenesis of human hepatocellular carcinoma. Nat Genet. 2002;31(4):339-346
30. Bayram S, Akkiz H, Bekar A. et al. The significance of Exonuclease 1 K589E polymorphism on hepatocellular carcinoma susceptibility in the Turkish population: a case-control study. Mol Biol Rep. 2012;39(5):5943-5951
31. Sumbul AT, Akkiz H, Bayram S. et al. p53 codon 72 polymorphism is associated with susceptibility to hepatocellular carcinoma in the Turkish population: a case-control study. Mol Biol Rep. 2012;39(2):1639-1647
32. Ning S, Bin C, Na H. et al. Glypican-3, a novel prognostic marker of hepatocellular cancer, is related with postoperative metastasis and recurrence in hepatocellular cancer patients. Mol Biol Rep. 2012;39(1):351-357
Corresponding author: Jian Liang, Department of Oncology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, NO. 10 Huadong Road, Nanning 530011, Guangxi Zhuang Autonomous Region, People's Republic of China. Tel: +86-771-2238026; Fax: +86-771-2238026 E-mail: jianliang_12com.