International Journal of Biological Sciences

Impact factor
3.873

ISSN 1449-2288

News feeds of IJBS published articles
My Manuscript
My Account

Journal of Biomedicinenew

Theranostics

International Journal of Medical Sciences

Journal of Cancer

Oncomedicine

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

Journal of Genomics now in PubMed/PubMed Central. Submit manuscript...

PubMed Central Indexed in Journal Impact Factor

Int J Biol Sci 2013; 9(4):343-349. doi:10.7150/ijbs.5501

Research Paper

Endosulfan-Induced Biomarkers in Japanese Rice Fish (Oryzias latipes) Analyzed by SELDI-TOF-MS

Sung-Eun Lee1,*, Choi Young-Woong2,*, Hyoung-ho Mo2, Jino Son2, Kyeonghun Park3, Kijong Cho2,✉

1. School of Applied Biosciences, Kyungpook National University, Daegu 702-701, South Korea;
2. Division of Environmental Sciences and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea;
3. Division of Pesticide Safety Evaluation, Department of Crop Life Safety, National Academy of Agriculture Science, Suwon 441-707, South Korea.
* Authors equally contributed to this work.

Abstract

The objective of this study was to find and validate estrogen-related biomarkers from plasma proteins in Oryzias latipes after exposure to an estrogen disrupting compound, α-endosulfan. The acute toxicity of α-endosulfan on O. latipes after 96 h of exposure was 13.72, 16.18, and 22.18 μg L-1 for the LC10, LC20, and LC50 values, respectively. To confirm estrogenic disturbance by α-endosulfan, the expression level of vitellogenin in the liver of male fishes was measured at the LC10 value, and it was found to be significantly different from the reference group, confirming the estrogenic effect of endosulfan in this concentration range. Proteinchip® array techniques using a weak cation exchange (CM10) and a strong anion exchange proteinchip (Q10) in conjunction with surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) were used to determine plasma proteins of O. latipes differently expressed in response to endosulfan exposure at LC10 and LC20 concentrations. Analysis of protein profiling of the male fish exposed to α-endosulfan detected 48 significantly different protein peaks and the proteins at m/z 2819, 8462, 8860, and 9462 were significantly different (p<0.05). The protein peaks at m/z 2819, 8860, and 9462 were up-regulated and the peak at m/z 8462 was down-regulated. Therefore, these four differentially expressed proteins could be used as biomarkers to rapidly determine a possible risk of endosulfan on aquatic ecosystems, although these are not necessarily produced as a result of endocrine disruption.

Keywords: Oryzias latipes, α-endosulfan, SELDI-TOF-MS, vitellogenin, biomarkers.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Lee SE, Young-Woong C, Mo Hh, Son J, Park K, Cho K. Endosulfan-Induced Biomarkers in Japanese Rice Fish (Oryzias latipes) Analyzed by SELDI-TOF-MS. Int J Biol Sci 2013; 9(4):343-349. doi:10.7150/ijbs.5501. Available from http://www.ijbs.com/v09p0343.htm