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Int J Biol Sci 2015; 11(4):361-369. doi:10.7150/ijbs.10858

Research Paper

PKD1 Mono-Allelic Knockout Is Sufficient to Trigger Renal Cystogenesis in a Mini-Pig Model

Jin He1,2, ✉, Qiuyan Li1, Suyun Fang1, Ying Guo1, Tongxin Liu1, Jianhua Ye1, Zhengquan Yu1, Ran Zhang1, Yaofeng Zhao1, Xiaoxiang Hu1, Xueyuan Bai3, Xiangmei Chen3, Ning Li1,4,✉

1. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China
2. College of Animal Science and Technology, China Agricultural University, Beijing, PR China
3. Department of Nephrology, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, PR China
4. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, PR China

Abstract

PKD1 and PKD2 mutations could lead to autosomal dominant polycystic kidney disease (ADPKD), which afflicts millions of people worldwide. Due to the marked differences in the lifespan, size, anatomy, and physiology from humans, rodent ADPKD models cannot fully mimic the disease. To obtain a large animal model that recapitulates the disease, we constructed a mini-pig model by mono-allelic knockout (KO) of PKD1 using zinc finger nuclease. The mono-allelic KO pigs had lower PKD1 expression than their wild-type littermates at both the transcriptional and translational levels. After approximately six months, renal cysts appeared and grew progressively in the KO pigs. Histological analysis showed that renal cysts were scatteredly distributed in the mutant pig kidneys and were lined by either cuboidal or flattened epithelial cells. Contrast-enhanced computed tomography confirmed that all of the mutant pigs had renal and hepatic cysts, when they were 11-month-old. Immunohistochemical analysis revealed that most of the cysts were derived from the proximal tubules and collecting ducts. Therefore, the PKD1 mono-allelic knockout is sufficient to trigger renal cystogenesis, and this pig model may provide a platform for future study of renal cyst formation.

Keywords: ADPKD, PKD1, ZFN, Disease model

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How to cite this article:
He J, Li Q, Fang S, Guo Y, Liu T, Ye J, Yu Z, Zhang R, Zhao Y, Hu X, Bai X, Chen X, Li N. PKD1 Mono-Allelic Knockout Is Sufficient to Trigger Renal Cystogenesis in a Mini-Pig Model. Int J Biol Sci 2015; 11(4):361-369. doi:10.7150/ijbs.10858. Available from http://www.ijbs.com/v11p0361.htm