Int J Biol Sci
2015; 11(10):1140-1149.
doi:10.7150/ijbs.12657 This issueCite
Research Paper
Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation
1. Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China 2. Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND, 58105, USA 3. Department of Neurology, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China 4. Department of Statistics, North Dakota State University, Fargo, ND, 58105, USA * Equal contribution
Wang X, Zhou S, Ding X, Ma M, Zhang J, Zhou Y, Wu E, Teng J. Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation. Int J Biol Sci 2015; 11(10):1140-1149. doi:10.7150/ijbs.12657. https://www.ijbs.com/v11p1140.htm
TAR DNA binding protein 43 (TDP-43) A315T mutation (TDP-43A315T) has been found in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) as a disease causing mutation with enhanced protein aggregation, formation of protease-resistant fragments, and neurotoxicity. However, the molecular mechanisms for its pathogenic effects are largely unknown. In this study, we demonstrate that TDP-43A315T enhanced neuronal toxicity via activating endoplasmic reticulum (ER) stress-mediated apoptosis in SH-SY5Y cells. Moreover, autophagy was activated by overexpression of TDP-43A315T in a self-defensive manner to decrease neuronal toxicity. Inhibition of autophagy attenuates TDP-43A315T induced neuronal cell death. Furthermore, the expression levels of TDP-43, ER chaperone 78 kDa glucose-regulated protein (GRP-78), and autophagy marker microtubule-associated protein 1A/1B-light chain 3 (LC3) in the skin tissues from ALS patients with TDP-43A315T mutation were markedly higher than those from the healthy control. Thus, our findings provide new molecular evidence for TDP-43A315T neuropathology. In addition, the pathological change in the skin tissues of the patients with TDP-43A315T mutation can be used as a quick diagnostic biomarker.
Wang, X., Zhou, S., Ding, X., Ma, M., Zhang, J., Zhou, Y., Wu, E., Teng, J. (2015). Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation. International Journal of Biological Sciences, 11(10), 1140-1149. https://doi.org/10.7150/ijbs.12657.
ACS
Wang, X.; Zhou, S.; Ding, X.; Ma, M.; Zhang, J.; Zhou, Y.; Wu, E.; Teng, J. Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation. Int. J. Biol. Sci. 2015, 11 (10), 1140-1149. DOI: 10.7150/ijbs.12657.
NLM
Wang X, Zhou S, Ding X, Ma M, Zhang J, Zhou Y, Wu E, Teng J. Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation. Int J Biol Sci 2015; 11(10):1140-1149. doi:10.7150/ijbs.12657. https://www.ijbs.com/v11p1140.htm
CSE
Wang X, Zhou S, Ding X, Ma M, Zhang J, Zhou Y, Wu E, Teng J. 2015. Activation of ER Stress and Autophagy Induced by TDP-43 A315T as Pathogenic Mechanism and the Corresponding Histological Changes in Skin as Potential Biomarker for ALS with the Mutation. Int J Biol Sci. 11(10):1140-1149.