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Int J Biol Sci 2017; 13(3):383-390. doi:10.7150/ijbs.17236

Research Paper

Inhibition of Proteasome Activity Induces Aggregation of IFIT2 in the Centrosome and Enhances IFIT2-Induced Cell Apoptosis

Limin Chen1,2#, Shuyuan Liu1#, Fen Xu3, Yunyuan Kong1, Lagen Wan1, Yonglu zhang1, Zhanglin Zhang1✉

1. Department of Clinical laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China;
2. Center for Experimental Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China;
3. Department of Clinical Laboratory Technology, Jiangxi Medical College, Shangrao, Jiangxi 334000, China.
#Contributed equally.

Abstract

IFN-induced protein with tetratricopeptide repeats 2 (IFIT2), one of the most highly responsive interferon-stimulated genes, inhibits the proliferation and migration of cancer cells and regulates viral replication. IFIT2 has been demonstrated to be a cytoskeleton-associated protein that becomes enriched in the mitotic spindle of cells. However, the molecular mechanisms by which IFIT2 executes biological functions are largely unclear. The findings of this study showed that inhibiting the activation of proteasome led to the enrichment of IFIT2 and induced the aggregation of IFIT2 protein in the centrosome. Microtubule inhibitor colchicine and dynein inhibitor ciliobrevin inhibited the proteasome inhibitor-induced aggregation of IFIT2 protein in the centrosome. Intriguingly, IFIT2 and proteasome inhibitor worked together to induce the apoptosis of cancer cells. The results of the present study revealed that the inhibition of proteasome activity blocked the degradation of IFIT2 and promoted the aggregation of IFIT2 in the centrosome, which in turn induced cell apoptosis. In short, IFIT2 may be a potential target for cancer therapeutics.

Keywords: Aggregation, apoptosis, centrosome, IFIT2, proteasome

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How to cite this article:
Chen L, Liu S, Xu F, Kong Y, Wan L, zhang Y, Zhang Z. Inhibition of Proteasome Activity Induces Aggregation of IFIT2 in the Centrosome and Enhances IFIT2-Induced Cell Apoptosis. Int J Biol Sci 2017; 13(3):383-390. doi:10.7150/ijbs.17236. Available from http://www.ijbs.com/v13p0383.htm