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Int J Biol Sci 2017; 13(7):923-934. doi:10.7150/ijbs.17260

Research Paper

Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress

Jianing Zhong1,#,✉, Liying Ji2, #, Huiqian Chen2, Xianfeng Li3, 4, Jian'an Zhang2, Xingxing Wang2, Weilin Wu1, Ying Xu1, Fei Huang1, Wanshi Cai3,4, Zhong Sheng Sun2,3,4,✉

1. The Science Research Center, Gannan Medical University, Ganzhou 341000, China
2. Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou 325035, China
3. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
4. University of Chinese Academy of Sciences, Beijing 100049, China
# Co-first author

Abstract

Oxidative stress is considered to be a key risk state for a variety of human diseases. In response to oxidative stress, the regulation of transcriptional expression of DNA repair genes would be important to DNA repair and genomic stability. However, the overall pattern of transcriptional expression of DNA repair genes and the underlying molecular response mechanism to oxidative stress remain unclear. Here, by employing colorectal cancer cell lines following exposure to hydrogen peroxide, we generated expression profiles of DNA repair genes via RNA-seq and identified gene subsets that are induced or repressed following oxidative stress exposure. RRBS-seq analyses further indicated that transcriptional regulation of most of the DNA repair genes that were induced or repressed is independent of their DNA methylation status. Our analyses also indicate that hydrogen peroxide induces deacetylase SIRT1 which decreases chromatin affinity and the activity of histone acetyltransferase hMOF toward H4K16ac and results in decreased transcriptional expression of DNA repair genes. Taken together, our findings provide a potential mechanism by which oxidative stress suppresses DNA repair genes which is independent of the DNA methylation status of their promoters.

Keywords: hMOF, H4K16ac, Oxidative Stress

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Zhong J, Ji L, Chen H, Li X, Zhang J, Wang X, Wu W, Xu Y, Huang F, Cai W, Sun ZS. Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress. Int J Biol Sci 2017; 13(7):923-934. doi:10.7150/ijbs.17260. Available from http://www.ijbs.com/v13p0923.htm