1. Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China. 2. GenoArray Biotech, Suzhou, China 3. Genex Health Co., Ltd, Beijing, China
✉ Corresponding author: Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China. Email: sdfyyliruicom
Citation:
You W, Zhang X, Ji M, Yu Y, Chen C, Xiong Y, Liu Y, Sun Y, Tan C, Zhang H, Li J, Chen W, Li R. MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells. Int J Biol Sci 2018; 14(6):644-653. doi:10.7150/ijbs.25272. https://www.ijbs.com/v14p0644.htm
Gastric cancer (GC) is one of the most common malignancies with high mortality rate. MiR-152 may exert the function of tumor suppressor by regulating its target gene, including PIK3CA. Nevertheless, all of the described functions are referred explicitly to miR-152-3p, while miR-152-5p as a passenger strand is poorly realized and entirely ignored. We previously selected miR-152-5p as a candidate using cell migration inhibition screening for GC cells and predicted that miR-152-5p might also target PIK3CA. In this study, we found an abnormal proportion of miR-152-3p / miR-152-5p in GC (gastric cancer) tissues and cells and demonstrated that miR-152-5p had poorer stability in GC cells, revealing the possibility that miR-152-5p is abnormally "suppressed" in gastric cancer. We also investigated and confirmed the role of miR-152-5p in GC by a series of experiments, and found that miR-152-5p modulated cell viability, migration, invasion, and cell-cycle progression of human GC cells, and also inhibited tumor growth and metastasis in vivo partially by targeting PIK3CA. More interestingly, it was proved that miR-152-3p and miR-152-5p had synergistic effects on the inhibition of PIK3CA in GC cells. The results of this study suggest that miR-152-5p may act as a tumor suppressor in SGC-7901 gastric cancer cells via targeting PIK3CA. Further, the study provides a novel insight into the roles of miRNA* during carcinogenesis.
You, W., Zhang, X., Ji, M., Yu, Y., Chen, C., Xiong, Y., Liu, Y., Sun, Y., Tan, C., Zhang, H., Li, J., Chen, W., Li, R. (2018). MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells. International Journal of Biological Sciences, 14(6), 644-653. https://doi.org/10.7150/ijbs.25272.
ACS
You, W.; Zhang, X.; Ji, M.; Yu, Y.; Chen, C.; Xiong, Y.; Liu, Y.; Sun, Y.; Tan, C.; Zhang, H.; Li, J.; Chen, W.; Li, R. MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells. Int. J. Biol. Sci. 2018, 14 (6), 644-653. DOI: 10.7150/ijbs.25272.
NLM
You W, Zhang X, Ji M, Yu Y, Chen C, Xiong Y, Liu Y, Sun Y, Tan C, Zhang H, Li J, Chen W, Li R. MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells. Int J Biol Sci 2018; 14(6):644-653. doi:10.7150/ijbs.25272. https://www.ijbs.com/v14p0644.htm
CSE
You W, Zhang X, Ji M, Yu Y, Chen C, Xiong Y, Liu Y, Sun Y, Tan C, Zhang H, Li J, Chen W, Li R. 2018. MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells. Int J Biol Sci. 14(6):644-653.
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