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Int J Biol Sci 2018; 14(10):1306-1320. doi:10.7150/ijbs.26829

Research Paper

Preliminary Observation about Alteration of Proteins and Their Potential Functions in Spinal Cord of SOD1 G93A Transgenic Mice

Jie Zhang1*, Ping Huang1,2*, Chengsi Wu1*, Huiting Liang1, Yue Li3, Lei Zhu1, Yi Lu1, Chunyan Tang1, Renshi Xu1✉

1. Department of Neurology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China
2. Department of Nutrition, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China
3. Department of Health Statistics, Nanchang University, Nanchang 330006, Jiangxi, China
* indicated the equal contributors

Abstract

The protein abnormality participates in the development of ALS that meets with the widespread approval from major researchers. However, these currently found abnormal proteins aren't far enough to explain all pathogenesis of ALS. Therefore, the search of novel abnormal proteins participated in the pathogenesis of ALS is very necessary. In this study, we screened, compared and analyzed the differentially expressed proteins in the spinal cord of the SOD1 G93A transgenic and wild-type (WT) mice applying the isobaric tags for relative and absolute quantitation (iTRAQ) and the bioinformatics methods. The results revealed the details of significantly differentially expressed proteins between the SOD1 G93A transgenic and WT mice, and the damaged and/or regulated cellular components, molecular functions and biological processes and the significant enrichment pathways of these proteins. Our study comprehensively described the details of the possible abnormal proteins participated in the pathogenesis of SOD1 G93A transgenic mice, extensively explored their possible molecular mechanisms how to play the role in the development in this animal model, and provided some evidences and clues for further and deeply studying the relationship between the abnormal proteins and the pathogenesis of ALS in the other animal models and ALS patients.

Keywords: Amyotrophic lateral sclerosis, Proteomics, iTRAQ, Gene ontology, Cluster of orthologous groups

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Zhang J, Huang P, Wu C, Liang H, Li Y, Zhu L, Lu Y, Tang C, Xu R. Preliminary Observation about Alteration of Proteins and Their Potential Functions in Spinal Cord of SOD1 G93A Transgenic Mice. Int J Biol Sci 2018; 14(10):1306-1320. doi:10.7150/ijbs.26829. Available from http://www.ijbs.com/v14p1306.htm