Int J Biol Sci 2019; 15(2):416-429. doi:10.7150/ijbs.26884

Research Paper

Small RNA sequencing reveals dynamic microRNA expression of important nutrient metabolism during development of Camellia oleifera fruit

Xiao-Xia Liu, Xiao-Fang Luo, Ke-Xin Luo, Ya-Lin Liu, Ting Pan, Zhi-Zhang Li, Gregory J. Duns, Fu-Lin He, Zuo-Dong Qin

Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Provincial Engineering Research Center for Ginkgo biloba, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425100, China

Abstract

To obtain insight into the function of miRNAs in the synthesis and storage of important nutrients during the development of Camellia oleifera fruit, Illumina sequencing of flower and fruit small-RNA was conducted. The results revealed that 797 miRNAs were significantly differentially expressed between flower and fruit samples of Camellia oleifera. Through integrated GO and KEGG function annotations, it was determined that the miRNA target genes were mainly involved in metabolic pathways, plant hormone signal transduction, fruit development, mitosis and regulation of biosynthetic processes. Carbohydrate accumulation genes were differentially regulated by miR156, miR390 and miR395 in the fruit growth and development process. MiR477 is the key miRNA functioning in regulation of genes and involved in fatty acid synthesis. Additionally, miR156 also has the function of regulating glycolysis and nutrient transformation genes.

Keywords: Camellia oleifera, deeping sequencing, microRNA, development of fruit, nutrient metabolism regulation

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How to cite this article:
Liu XX, Luo XF, Luo KX, Liu YL, Pan T, Li ZZ, Duns GJ, He FL, Qin ZD. Small RNA sequencing reveals dynamic microRNA expression of important nutrient metabolism during development of Camellia oleifera fruit. Int J Biol Sci 2019; 15(2):416-429. doi:10.7150/ijbs.26884. Available from http://www.ijbs.com/v15p0416.htm