The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer

Wu, Guiyang; Yuan, Shichao; Chen, Zaiping; Chen, Guoping; Fan, Qinghao; Dong, Hao; Ye, Fubo; Li, Jing; Zhu, Xiongwen

doi:10.7150/ijbs.30652

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Int J Biol Sci 2019; 15(3):628-635. doi:10.7150/ijbs.30652 This issue Cite

Research Paper

The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer

Guiyang Wu^1*, Shichao Yuan^1*, Zaiping Chen¹, Guoping Chen¹, Qinghao Fan¹, Hao Dong¹, Fubo Ye^1✉, Jing Li^2✉, Xiongwen Zhu^1✉

1. Department of General Surgery, Taizhou Municipal Hospital, Medical School of Taizhou University, Taizhou 318000, Zhejiang Province, China.
2. Departments of CyberKnife, Huashan Hospital, Fudan University, Shanghai 200032, China
*Guiyang Wu and Shichao Yuan contribute equally to this work.

✉ Corresponding authors: Fubo Ye, Department of General Surgery, Taizhou Municipal Hospital, Medical School of Taizhou University. No.318, East Zhongshan Road, Jiaojiang District, Taizhou 318000, China. Tel: 86-0576-88858024; Fax: 86-0576-88858024; E- More

Citation:

Wu G, Yuan S, Chen Z, Chen G, Fan Q, Dong H, Ye F, Li J, Zhu X. The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. Int J Biol Sci 2019; 15(3):628-635. doi:10.7150/ijbs.30652. https://www.ijbs.com/v15p0628.htm

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Abstract

The Krüppel-like transcription factor 14 (KLF14) is a critical regulator of a wide array of biological processes. However, the role of KLF14 in colorectal cancer (CRC) isn't fully investigated. This study aimed to explore the clinicopathological significance and potential role of KLF14 in the carcinogenesis and progression of CRC. A tissue microarray consisting of 185 samples from stage I-III CRC patients was adopted to analyze the correlation between KLF14 expression and clinicopathological parameters, as well as overall survival (OS) and disease-free survival (DFS). The underlying mechanisms of altered KLF14 expression on glycolysis were studied using in vitro and patients' samples. The results showed that KLF14 expression was downregulated in CRC than their normal controls. Low KLF14 expression correlated with advanced T stage (P< 0.001) and N stage (P= 0.040), and larger tumor size (P= 0.008). Lost KLF14 expression implied shorter OS and DFS after colectomy in both univariate and multivariate survival analysis (P<0.05). Experimentally, restore KLF14 expression significantly decreased the rate of glycolysis both in vitro and in patients' sample. Mechanically, KLF14 regulated glycolysis by downregulating glycolytic enzyme LDHB. Collectively, KLF14 is a novel prognostic biomarker for survival in CRC, and downregulation of KLF14 in CRC prompts glycolysis by target LDHB. Hence, KLF14 could constitute potential prognostic predictors and therapeutic targets for CRC.

Keywords: Colorectal Cancer, Glycolysis, KLF14, LDHB

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APA

Wu, G., Yuan, S., Chen, Z., Chen, G., Fan, Q., Dong, H., Ye, F., Li, J., Zhu, X. (2019). The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. International Journal of Biological Sciences, 15(3), 628-635. https://doi.org/10.7150/ijbs.30652.

ACS

Wu, G.; Yuan, S.; Chen, Z.; Chen, G.; Fan, Q.; Dong, H.; Ye, F.; Li, J.; Zhu, X. The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. Int. J. Biol. Sci. 2019, 15 (3), 628-635. DOI: 10.7150/ijbs.30652.

NLM

CSE

Wu G, Yuan S, Chen Z, Chen G, Fan Q, Dong H, Ye F, Li J, Zhu X. 2019. The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. Int J Biol Sci. 15(3):628-635.

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