Int J Biol Sci 2019; 15(8):1743-1754. doi:10.7150/ijbs.34820

Research Paper

Generation of Tβ4 knock-in Cashmere goat using CRISPR/Cas9

Xiaocong Li*, Fei Hao*, Xiao Hu, Hui Wang, Bai Dai, Xiao Wang, Hao Liang, Ming Cang, Dongjun Liu

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010000, China.
*These authors contributed equally to this work.


The cashmere goat breed is known to provide excellent quality cashmere. Here, we attempted to breed high-yielding cashmere goats by specifically inserting the Tβ4 gene into the goat CCR5 locus and provided an animal model for future research. We successfully obtained Tβ4 knock-in goat without any screening and fluorescent markers using CRISPR/Cas9 technology. A series of experiments were performed to examine physical conditions and characteristics of the Tβ4 knock-in goat. The goat exhibited an increase in cashmere yield by 74.5% without affecting the fineness and quality. Additionally, RNA-seq analysis indicated that Tβ4 may promote hair growth by affecting processes such as vasoconstriction, angiogenesis, and vascular permeability around secondary hair follicles. Together, our study can significantly improve the breeding of cashmere goat and thereby increase economic efficiency.

Keywords: CRISPR/Cas9, Tβ4, goat, knock-in, hair growth

This is an open access article distributed under the terms of the Creative Commons Attribution License ( See for full terms and conditions.
How to cite this article:
Li X, Hao F, Hu X, Wang H, Dai B, Wang X, Liang H, Cang M, Liu D. Generation of Tβ4 knock-in Cashmere goat using CRISPR/Cas9. Int J Biol Sci 2019; 15(8):1743-1754. doi:10.7150/ijbs.34820. Available from