Int J Biol Sci 2020; 16(1):85-98. doi:10.7150/ijbs.33976 This issue Cite

Research Paper

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction

Wenbo Liu, Kang Wang, Xiaoyan Lv, Qian Wang, Xiu Li, Zhigang Yang, Xia Liu, Li Yan, Xin Fu, Ran Xiao

Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba-Da-Chu Road, Beijing, 100144, People's Republic of China

Citation:
Liu W, Wang K, Lv X, Wang Q, Li X, Yang Z, Liu X, Yan L, Fu X, Xiao R. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 2020; 16(1):85-98. doi:10.7150/ijbs.33976. https://www.ijbs.com/v16p0085.htm
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Abstract

Graphic abstract

Folate deficiency has long been associated with the abnormal development of the neural crest cells (NCCs) and neural tube defects (NTDs). RNA binding proteins (RBPs) also play important roles in the normal neural crest development and neural tube formation. Nevertheless, the causative mechanism by which folate status influences human NCCs development and the RBPs functions remains unknown. In this study, we differentiated H9 human embryonic stem cells into neural crest cells (H9-NCCs) and then constructed three folic acid (FA) deficiency (FAD) H9-NCCs models in vitro. Decreased viability, impaired migration and promoted apoptosis of H9-NCCs were observed in three FAD H9-NCCs models. In addition, we showed that three RBPs, namely, hnRNPC, LARP6 and RCAN2, were up-regulated both in the FAD H9-NCC models in vitro and in the FAD mouse model in vivo. Knocking down of these three RBPs increased the H9-NCC viability and RCAN2 knockdown further promoted H9-NCC migration under FAD conditions. In normal culture condition, overexpression of RCAN2 and HnRNPC did not affect viabilities and migration of H9-NCCs while overexpression of LARP6 reduced the H9-NCC viability. Our findings demonstrate important regulatory effects of RBPs underlying FAD-induced impaired function of NCCs.

Keywords: Neural crest cells, neural tube defects, folic acid deficiency, RNA binding protein


Citation styles

APA
Liu, W., Wang, K., Lv, X., Wang, Q., Li, X., Yang, Z., Liu, X., Yan, L., Fu, X., Xiao, R. (2020). Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. International Journal of Biological Sciences, 16(1), 85-98. https://doi.org/10.7150/ijbs.33976.

ACS
Liu, W.; Wang, K.; Lv, X.; Wang, Q.; Li, X.; Yang, Z.; Liu, X.; Yan, L.; Fu, X.; Xiao, R. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int. J. Biol. Sci. 2020, 16 (1), 85-98. DOI: 10.7150/ijbs.33976.

NLM
Liu W, Wang K, Lv X, Wang Q, Li X, Yang Z, Liu X, Yan L, Fu X, Xiao R. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 2020; 16(1):85-98. doi:10.7150/ijbs.33976. https://www.ijbs.com/v16p0085.htm

CSE
Liu W, Wang K, Lv X, Wang Q, Li X, Yang Z, Liu X, Yan L, Fu X, Xiao R. 2020. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci. 16(1):85-98.

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