Int J Biol Sci 2012; 8(10):1335-1344. doi:10.7150/ijbs.4654
Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice
1. Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
Intra bone marrow-bone marrow transplantation (IBM- BMT) + thymus transplantation (TT) has been shown to reduce the incidence of graft versus host disease (GVHD) and restore donor-derived T cell function. In addition, an increase in insulin sensitivity occurred in db/db mice after IBM-BMT+TT treatment. Heme oxygenase (HO)-1 is a stress inducible enzyme which exert antioxidant, antiapoptotic, and immune-modulating properties. We examined whether IBM-BMT+TT could modulate the expression of HO-1 in the kidneys of db/db mice. Six-week-old db/db mice with blood glucose levels higher than 250 mg/dl were treated with IBM-BMT+TT. Six weeks later, the db/db mice showed decreased body weight, blood glucose levels and insulin, and increased plasma adiponectin levels. The upregulation of HO-1 was associated with significantly (p<0.05) increased levels of peNOS and pAKT, but decreased levels of iNOS in the kidneys of db/db mice. Plasma creatinine levels also decreased (p<0.05), and the expression of type IV collagen was improved. Thus IBM-BMT+TT unregulated the expression of HO-1, peNOS and pAKT, while decreasing iNOS levels in the kidney of db/db mice. This was associated with an improvement in renal function.
Keywords: IBM-BMT+TT, antioxidant, HO-1, diabetic nephropathy.
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How to cite this article:
Li M, Vanella L, Zhang Y, Shi M, Takaki T, Shapiro JI, Ikehara S. Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice. Int J Biol Sci 2012; 8(10):1335-1344. doi:10.7150/ijbs.4654. Available from http://www.ijbs.com/v08p1335.htm