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Int J Biol Sci 2013; 9(4):382-390. doi:10.7150/ijbs.5510

Research Paper

Dpysl4 Is Involved in Tooth Germ Morphogenesis through Growth Regulation, Polarization and Differentiation of Dental Epithelial Cells

Masato Yasukawa1, Kentaro Ishida2, Yohei Yuge1, Mai Hanaoka1, Yoko Minami1, Miho Ogawa2,3, Takashi Sasaki2, Masahiro Saito1,2, Takashi Tsuji1,2,3,✉

1. Department of Biological Science and Technology, Graduate school of Industrial Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan;
2. Research Institute for Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan;
3. Organ Technologies Inc., Tokyo 101-0048, Japan.

Abstract

Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.

Keywords: Development, cell polarization, tooth germ, differentiation, Dpysl4.

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How to cite this article:
Yasukawa M, Ishida K, Yuge Y, Hanaoka M, Minami Y, Ogawa M, Sasaki T, Saito M, Tsuji T. Dpysl4 Is Involved in Tooth Germ Morphogenesis through Growth Regulation, Polarization and Differentiation of Dental Epithelial Cells. Int J Biol Sci 2013; 9(4):382-390. doi:10.7150/ijbs.5510. Available from http://www.ijbs.com/v09p0382.htm