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Int J Biol Sci 2006; 2(1):23-29. doi:10.7150/ijbs.2.23

Short Research Communication

Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility

Yao-Yuan Hsieh1 2, Chi-Chen Chang1, Chich-Sheng Lin2

1. Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan
2. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan


Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters.

Patients and methods: Specimens were divided into two groups: group 1. normospermia (n=20); group 2. oligoasthenospermia (n=31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared.

Results: MDA concentrations in both groups were significantly different (1.52 ± 0.75 vs. 2.25 ± 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 ± 0.11 vs. 0.47 ± 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p =0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility.

Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.

Keywords: glutathione peroxidase, malondialdehyde, oligoasthenozoospermia, semen, sperm

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How to cite this article:
Hsieh YY, Chang CC, Lin CS. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int J Biol Sci 2006; 2(1):23-29. doi:10.7150/ijbs.2.23. Available from