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Int J Biol Sci 2008; 4(6):379-386. doi:10.7150/ijbs.4.379

Research Paper

Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713

Farwa Sarwat1, Shah Ali Ul Qader2 ✉, Afsheen Aman3, Nuzhat Ahmed1

1. Centre for Molecular Genetics, University of Karachi, Pakistan
2. Institute of Sustainable Halophytes Utilization, University of Karachi-75270, Pakistan
3. Pharmaceutical Research Centre, PCSIR Laboratories complex, Karachi, Pakistan

Abstract

On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced maximum dextran after 20 hours of incubation at 30ºC with 15% sucrose at pH 7.0. The molecular mass distribution of dextran produced by this strain showed that its molecular mass was about 2.0 million Da. Dextran analysis by 13C-NMR spectrometry showed no signals corresponding to any other linkages except α-(1→6) glycosidic linkage in the main chain, which has not been reported before. Physico-chemical properties of this unique dextran were also studied. These optimised conditions could be used for the commercial production of this unique high molecular weight dextran, which have significant industrial perspectives.

Keywords: L. mesenteroides, Dextran, NMR

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How to cite this article:
Sarwat F, Qader SAU, Aman A, Ahmed N. Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713. Int J Biol Sci 2008; 4(6):379-386. doi:10.7150/ijbs.4.379. Available from http://www.ijbs.com/v04p0379.htm