International Journal of Biological Sciences

Impact factor
3.873

ISSN 1449-2288

News feeds of IJBS published articles
My Manuscript
My Account

Journal of Biomedicinenew

Theranostics

International Journal of Medical Sciences

Journal of Cancer

Oncomedicine

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

Journal of Genomics now in PubMed/PubMed Central. Submit manuscript...

PubMed Central Indexed in Journal Impact Factor

Int J Biol Sci 2010; 6(1):80-88. doi:10.7150/ijbs.6.80

Short Research Communication

Cellular and molecular comparison of redifferentiation of intramuscular- and visceral-adipocyte derived progeny cells

Jie Chen1,2 , Michael V. Dodson2, Zhihua Jiang2

1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA

Abstract

In the present study, mature adipocytes from pig-derived visceral and intramuscular adipose depots were isolated, purified, and allowed to undergo dedifferentiation and redifferentiation in vitro. During the redifferentiation process at days 1, 2, 4, 6, and 8, we observed that both visceral- and intramuscular adipose-derived progeny cells possessed a similar capacity to accumulate lipid. However, at days 10, 12, 14, and 16, the latter progeny cells accumulated lipid much faster--the content almost doubled at day 16 (P < 0.05). Such faster potential of lipid accumulation in the intramuscular adipose-derived progeny cells was then supported by higher expressions of CCAAT/enhancer binding protein-α (CEBP-α) and peroxisome proliferator-activated receptor-γ (PPAR-γ) at all these nine time points, and diacylglycerol O-acyltransferase homolog 1 (DGAT1), fatty acid binding protein 4 (FABP4) and fatty acid synthase (FASN) at some time points (P < 0.05). These preliminary data suggest that adipose depot differences exist with respect to ability of purified cells of the adipose lineage to redifferentiate and form viable lipid-assimilating cells in vitro. Therefore, our present study might provide a foundation to develop tools for biomedical and agricultural applications, as well as to determine the regulation of depot-specific cells of the adipose lineage. Further studies with more animals will validate and expand our results.

Keywords: Visceral adipocytes, Intramuscular adipocytes, Dedifferentiation, Redifferentiation, Gene expression dynamics.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Chen J, Dodson MV, Jiang Z. Cellular and molecular comparison of redifferentiation of intramuscular- and visceral-adipocyte derived progeny cells. Int J Biol Sci 2010; 6(1):80-88. doi:10.7150/ijbs.6.80. Available from http://www.ijbs.com/v06p0080.htm