Int J Biol Sci 2010; 6(7):834-844. doi:10.7150/ijbs.6.834
Microbial and Bioconversion Production of D-xylitol and Its Detection and Application
1. Biorefining Research Initiative and Department of Biology, Lakehead University, Thunder Bay, ON, P7B 5E1, Canada
D-Xylitol is found in low content as a natural constituent of many fruits and vegetables. It is a five-carbon sugar polyol and has been used as a food additive and sweetening agent to replace sucrose, especially for non-insulin dependent diabetics. It has multiple beneficial health effects, such as the prevention of dental caries, and acute otitis media. In industry, it has been produced by chemical reduction of D-xylose mainly from photosynthetic biomass hydrolysates. As an alternative method of chemical reduction, biosynthesis of D-xylitol has been focused on the metabolically engineered Saccharomyces cerevisiae and Candida strains. In order to detect D-xylitol in the production processes, several detection methods have been established, such as gas chromatography (GC)-based methods, high performance liquid chromatography (HPLC)-based methods, LC-MS methods, and capillary electrophoresis methods (CE). The advantages and disadvantages of these methods are compared in this review.
Keywords: D-xylitol, Bioconversion production, Detection methods, Saccharomyces cerevisiae, Candida.
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Chen X, Jiang ZH, Chen S, Qin W. Microbial and Bioconversion Production of D-xylitol and Its Detection and Application. Int J Biol Sci 2010; 6(7):834-844. doi:10.7150/ijbs.6.834. Available from http://www.ijbs.com/v06p0834.htm