International Journal of Biological Sciences

Impact factor

ISSN 1449-2288

News feeds of IJBS published articles
Manuscript login

open access Global reach, higher impact

Journal of Genomics in PubMed Central. Submit manuscript now...


International Journal of Medical Sciences

Journal of Cancer

Journal of Genomics


PubMed Central Indexed in Journal Impact Factor

Int J Biol Sci 2011; 7(1):133-137. doi:10.7150/ijbs.7.133

Short Research Communication

MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines

Yu Deng1,4#, Hui Deng2,3,4#, Feng Bi1, Jing Liu4, Lynn T. Bemis5, David Norris4, Xiao-Jing Wang3, Qinghong Zhang3,4

1. Laboratory of Signal Transduction & Molecular Targeted Therapy, State Key Laboratory of Biotherapy/ Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, 610041, China;
2. Department of Dermatology, The Sixth People's Hospital of Shanghai, Shanghai Jiaotong University, Yishan Road 600, Shanghai, 200211, China;
3. Department of Pathology, University of Colorado, Denver, Aurora, CO 80045, USA;
4. Department of Dermatology, University of Colorado, Denver, Aurora, CO 80045, USA;
5. Department of Medicine, University of Colorado, Denver, Aurora, CO 80045, USA.
# Co-first author


Carboxyl-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that represses expression of various tumor suppressor genes. In the present study, we identified miR-137 as a potential regulator of CtBP1 expression in melanoma cells. Expression of miR-137 in melanoma cell lines was found to inversely correlate with CtBP1 levels. Target Scan predicted a putative site for miR-137 within the CtBP1 3′ untranslated region (3′UTR) at nt 710-716, which is highly conserved across species. To explore the mechanism of miR-137 targeting CtBP1, we performed an Argonaute 2 (Ago2)-pull down assay, and miR-137 was identified in complex with CtBP1 mRNA. miR-137 suppressed CtBP1 3' UTR luciferase-reporter activity, and this effect was lost with deletion of the putative 3' UTR target-site. Consistent with the results of the reporter assay, ectopic expression of miR-137 reduced expression levels of CtBP1. Furthermore, expression of miR-137 increased the immediate downstream effectors of CtBP1, such as E-cadherin and Bax. The human miR-137 gene is located at chromosome 1p22, which has previously been determined to be a susceptive region for melanoma. This study suggests miR-137 may act as a tumor suppressor by directly targeting CtBP1 to inhibit epithelial-mesenchymal transition (EMT) and inducing apoptosis of melanoma cells, thus illustrating a functional link between miR-137 and CtBP1 in melanoma development.

Keywords: CtBP1, miR-137, transcription, tumor suppressor, melanoma

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See for full terms and conditions.
How to cite this article:
Deng Y, Deng H, Bi F, Liu J, Bemis LT, Norris D, Wang XJ, Zhang Q. MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines. Int J Biol Sci 2011; 7(1):133-137. doi:10.7150/ijbs.7.133. Available from