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Int J Biol Sci 2012; 8(4):498-511. doi:10.7150/ijbs.3723

Research Paper

Characterization of Bovine Induced Pluripotent Stem Cells by Lentiviral Transduction of Reprogramming Factor Fusion Proteins

Hongguo Cao, Pan Yang, Yong Pu, Xueping Sun, Huiqun Yin, Yu Zhang, Yunhai Zhang, Yunsheng Li, Ya Liu, Fugui Fang, Zijun Zhang, Yong Tao, Xiaorong Zhang

Anhui Local Livestock Genetic Resources Conservation and Breeding Laboratory, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Abstract

Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.

Keywords: bovine, fetal fibroblasts, iPS cells, defined-factor fusion proteins

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Cao H, Yang P, Pu Y, Sun X, Yin H, Zhang Y, Zhang Y, Li Y, Liu Y, Fang F, Zhang Z, Tao Y, Zhang X. Characterization of Bovine Induced Pluripotent Stem Cells by Lentiviral Transduction of Reprogramming Factor Fusion Proteins. Int J Biol Sci 2012; 8(4):498-511. doi:10.7150/ijbs.3723. Available from http://www.ijbs.com/v08p0498.htm