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Int J Biol Sci 2012; 8(4):512-521. doi:10.7150/ijbs.3641

Research Paper

Propolis Standardized Extract (EPP-AF®), an Innovative Chemically and Biologically Reproducible Pharmaceutical Compound for Treating Wounds

Andresa Aparecida Berretta1,2,✉, Andresa Piacezzi Nascimento2, Paula Carolina Pires Bueno3, Mirela Mara de Oliveira Lima Leite Vaz2, Juliana Maldonado Marchetti1

1. Laboratório de Tecnologia Farmacêutica, Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14043-903, Ribeirão Preto, SP, Brazil.
2. Laboratório de Pesquisa, Desenvolvimento & Inovação, Apis Flora Indl. Coml. Ltda., Rua Triunfo, 945, 14020-670, Ribeirão Preto, SP, Brazil.
3. Instituto de Química, Universidade Estadual Paulista, Rua Francisco Degni, s/n, CEP 14800-900, Araraquara, SP, Brazil.


The aim of this study was to develop a formulation, containing the propolis standardized extract (EPP-AF®), which can assist in the healing of skin lesions. To achieve this objective the antimicrobial activity and chemical composition of the propolis extract was determined. The final product was subjected to in vitro and in vivo pre-clinical evaluation. The broth macrodilution method was used to determine the antimicrobial activity of the extracts and formulations against the microorganisms most commonly found in burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. Wistar rats with puncture wounded skin were used to evaluate the wound healing properties of propolis. The results of chemical and biological characterization demonstrated the batch-to-batch reproducibility of the standardized extract which is an unprecedented result. The antimicrobial and wound healing activity of the pharmaceutical studied showed the best results when samples contain 3.6% propolis, suggesting that this is the most promising composition.

Keywords: propolis, EPP-AF®, antimicrobial activity, wound healing, pharmaceutics, reproducibility

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See for full terms and conditions.
How to cite this article:
Berretta AA, Nascimento AP, Bueno PCP, de Oliveira Lima Leite Vaz MM, Marchetti JM. Propolis Standardized Extract (EPP-AF®), an Innovative Chemically and Biologically Reproducible Pharmaceutical Compound for Treating Wounds. Int J Biol Sci 2012; 8(4):512-521. doi:10.7150/ijbs.3641. Available from