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Int J Biol Sci 2012; 8(8):1142-1155. doi:10.7150/ijbs.4588

Research Paper

Tissue-Specific Transcriptome Profiling of Plutella Xylostella Third Instar Larval Midgut

Wen Xie1*, Yanyuan Lei1*, Wei Fu1, Zhongxia Yang1, Xun Zhu1, Zhaojiang Guo1, Qingjun Wu1, Shaoli Wang1, Baoyun Xu1, Xuguo Zhou2✉, Youjun Zhang1✉

1. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
2. Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA.
* These authors contributed equally.

Abstract

The larval midgut of diamondback moth, Plutella xylostella, is a dynamic tissue that interfaces with a diverse array of physiological and toxicological processes, including nutrient digestion and allocation, xenobiotic detoxification, innate and adaptive immune response, and pathogen defense. Despite its enormous agricultural importance, the genomic resources for P. xylostella are surprisingly scarce. In this study, a Bt resistant P. xylostella strain was subjected to the in-depth transcriptome analysis to identify genes and gene networks putatively involved in various physiological and toxicological processes in the P. xylostella larval midgut.

Using Illumina deep sequencing, we obtained roughly 40 million reads containing approximately 3.6 gigabases of sequence data. De novo assembly generated 63,312 ESTs with an average read length of 416bp, and approximately half of the P. xylostella sequences (45.4%, 28,768) showed similarity to the non-redundant database in GenBank with a cut-off E-value below 10-5. Among them, 11,092 unigenes were assigned to one or multiple GO terms and 16,732 unigenes were assigned to 226 specific pathways. In-depth analysis indentified genes putatively involved in insecticide resistance, nutrient digestion, and innate immune defense. Besides conventional detoxification enzymes and insecticide targets, novel genes, including 28 chymotrypsins and 53 ABC transporters, have been uncovered in the P. xylostella larval midgut transcriptome; which are potentially linked to the Bt toxicity and resistance. Furthermore, an unexpectedly high number of ESTs, including 46 serpins and 7 lysozymes, were predicted to be involved in the immune defense.

As the first tissue-specific transcriptome analysis of P. xylostella, this study sheds light on the molecular understanding of insecticide resistance, especially Bt resistance in an agriculturally important insect pest, and lays the foundation for future functional genomics research. In addition, current sequencing effort greatly enriched the existing P. xylostella EST database, and makes RNAseq a viable option in the future genomic analysis.

Keywords: Illumina sequencing, expressed sequence tag, Plutella xylostella, midgut, insecticide resistance

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Xie W, Lei Y, Fu W, Yang Z, Zhu X, Guo Z, Wu Q, Wang S, Xu B, Zhou X, Zhang Y. Tissue-Specific Transcriptome Profiling of Plutella Xylostella Third Instar Larval Midgut. Int J Biol Sci 2012; 8(8):1142-1155. doi:10.7150/ijbs.4588. Available from http://www.ijbs.com/v08p1142.htm