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Int J Biol Sci 2013; 9(7):743-752. doi:10.7150/ijbs.5345

Research Paper

Elevated miR-34c-5p Mediates Dermal Fibroblast Senescence by Ultraviolet Irradiation

Bing-rong ZHOU*, Xian-fei GUO*, Jia-an ZHANG, Yang XU, Wei LI, Di WU, Zhi-qiang YIN, Felicia Permatasari, Dan LUO

Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Guangzhou road 300#, Nanjing, Jiangsu province, China PR.
* contribute equally to the paper.

Abstract

Previous studies showed that several miRNAs can regulate pathways involved in UVB-induced premature senescence and response to ultraviolet irradiation. It has also been reported that miR-34c-5p may be involved in senescence-related mechanisms. We propose that miR-34c-5p may play a crucial role in senescence of normal human primary dermal fibroblasts. Here, we explored the roles of miR-34c-5p in UVB-induced premature senescence on dermal fibroblasts. MiR-34c-5p expression was increased in dermal fibroblasts after repeated subcytotoxic UVB treatments. Underexpression of miR-34c-5p in dermal fibroblasts led to a marked delay of many senescent phenotypes induced by repeated UVB treatments. Furthermore, underexpression of miR-34c-5p in dermal fibroblasts can antagonize the alteration of G1-arrested fibroblasts. Moreover, E2F3, which can inactivate p53 pathway and play a role in cell cycle progression, is a down-stream target of miR-34c-5p. Forced down-expression of miR-34c-5p decreased the expression of UVB-SIPS induced P21 and P53 at both mRNA and protein levels. Our data demonstrated that down-regulation of miR-34c-5p can protect human primary dermal fibroblasts from UVB-induced premature senescence via regulations of some senescence-related molecules.

Keywords: miR-34c-5p, UVB, premature senescence, human skin fibroblasts.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
ZHOU Br, GUO Xf, ZHANG Ja, XU Y, LI W, WU D, YIN Zq, Permatasari F, LUO D. Elevated miR-34c-5p Mediates Dermal Fibroblast Senescence by Ultraviolet Irradiation. Int J Biol Sci 2013; 9(7):743-752. doi:10.7150/ijbs.5345. Available from http://www.ijbs.com/v09p0743.htm