International Journal of Biological Sciences

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Int J Biol Sci 2014; 10(1):90-102. doi:10.7150/ijbs.7738

Research Paper

Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells

Yongzhi Wang, Zhonghua Yang, Zhe Meng, Hong Cao, Guangbin Zhu, Tao Liu, Xinghuan Wang

Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, China, 430071

Abstract

As the function of transient receptor potential melastatin member 8 (TRPM8) in osteosarcoma is still unknown, we aim to investigate the possible effects and potential mechanisms of TRPM8 on cell proliferation, metastasis and chemosensitivity in osteosarcoma cells. We find that TRPM8 is aberrantly over-expressed in human osteosarcoma tissues and cell lines. Knockdown of TRPM8 by siRNA in osteosarcoma cells leads to the impaired regulation of intracellular Ca2+ concentration and then the Akt-GSK-3β pathway and the phosphorylation of p44/p42 and FAK are suppressed. Knockdown of TRPM8 not only negatively influences the cell proliferation and metastasis but also enhances epirubicin-induced cell apoptosis. Such results reveal that TRPM8 is worthy further investigation for its potential as a clinical biomarker and therapeutic target in osteosarcoma.

Keywords: TRPM8, osteosarcoma, epirubicin, apoptosis, MAPK

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How to cite this article:
Wang Y, Yang Z, Meng Z, Cao H, Zhu G, Liu T, Wang X. Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells. Int J Biol Sci 2014; 10(1):90-102. doi:10.7150/ijbs.7738. Available from http://www.ijbs.com/v10p0090.htm