Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells

Wang, Yongzhi; Yang, Zhonghua; Meng, Zhe; Cao, Hong; Zhu, Guangbin; Liu, Tao; Wang, Xinghuan

doi:10.7150/ijbs.7738

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Theranostics

International Journal of Medical Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

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Int J Biol Sci 2014; 10(1):90-102. doi:10.7150/ijbs.7738 This issue Cite

Research Paper

Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells

Yongzhi Wang, Zhonghua Yang, Zhe Meng, Hong Cao, Guangbin Zhu, Tao Liu, Xinghuan Wang^✉

Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, China, 430071

Citation:

Wang Y, Yang Z, Meng Z, Cao H, Zhu G, Liu T, Wang X. Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells. Int J Biol Sci 2014; 10(1):90-102. doi:10.7150/ijbs.7738. https://www.ijbs.com/v10p0090.htm

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Abstract

As the function of transient receptor potential melastatin member 8 (TRPM8) in osteosarcoma is still unknown, we aim to investigate the possible effects and potential mechanisms of TRPM8 on cell proliferation, metastasis and chemosensitivity in osteosarcoma cells. We find that TRPM8 is aberrantly over-expressed in human osteosarcoma tissues and cell lines. Knockdown of TRPM8 by siRNA in osteosarcoma cells leads to the impaired regulation of intracellular Ca²⁺ concentration and then the Akt-GSK-3β pathway and the phosphorylation of p44/p42 and FAK are suppressed. Knockdown of TRPM8 not only negatively influences the cell proliferation and metastasis but also enhances epirubicin-induced cell apoptosis. Such results reveal that TRPM8 is worthy further investigation for its potential as a clinical biomarker and therapeutic target in osteosarcoma.

Keywords: TRPM8, osteosarcoma, epirubicin, apoptosis, MAPK

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APA

Wang, Y., Yang, Z., Meng, Z., Cao, H., Zhu, G., Liu, T., Wang, X. (2014). Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells. International Journal of Biological Sciences, 10(1), 90-102. https://doi.org/10.7150/ijbs.7738.

ACS

Wang, Y.; Yang, Z.; Meng, Z.; Cao, H.; Zhu, G.; Liu, T.; Wang, X. Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells. Int. J. Biol. Sci. 2014, 10 (1), 90-102. DOI: 10.7150/ijbs.7738.

NLM

CSE

Wang Y, Yang Z, Meng Z, Cao H, Zhu G, Liu T, Wang X. 2014. Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells. Int J Biol Sci. 10(1):90-102.

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