Int J Biol Sci 2014; 10(2):136-141. doi:10.7150/ijbs.7515
Micrometastasis Expressing Insulin Arise in Lung and Spleen at Advanced Stage of Rip1-Tag2 Transgenic Mice
1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China;
Rip1-Tag2 mice is one overt pancreatic β-cell tumor model, which is widely used for studying pancreas tumor angiogenesis and tumor development. However, tumor metastasis in Rip1-Tag2 mice had rarely been reported, in this present study, we find some micrometastasis in lung and spleen of the Rip1-Tag2 mice at advanced stage, which is important for uncovering metastasis cell characteristics and exploring how to survive in cancer microenvironment. To study the micrometastasis of Rip1-Tag2 mice in advanced pancreatic cancer, we first observed the pathology process of β cell tumor in Rip1-Tag2 mice through HE staining, then we performed immunohistochemistry with insulin antibody, T-antigen antibodies and C-petide antibody on lung and spleen tissues sections from advanced stage, comparing with background wild-type C57BL/6 mice sections. The results indicated that micrometastasis expressing insulin was found in the Rip1-Tag2 mice lung, and spleen. Further evidences demonstrate pathology structure of lung and spleen are damaged. Interestingly and importantly, the expression of T antigen and insulin antibodies are all decreased in advanced stage of primary β cell tumor, which suggest that the at least partly micrometastasis is derived from the early stage or from advanced stage of β cell tumor then return to undifferentiated state like cancer stem cell. The findings contributed to the study of cancer metastasis and cancer stem cell.
Keywords: micrometastasis, insulin, Rip1-Tag2 transgenic mice
Li Jc, Yang Y, Zhou Xm, Guo Sm, Liu Y, Qi Cl, Lan T, Zhang Qq, Wang Lj. Micrometastasis Expressing Insulin Arise in Lung and Spleen at Advanced Stage of Rip1-Tag2 Transgenic Mice. Int J Biol Sci 2014; 10(2):136-141. doi:10.7150/ijbs.7515. Available from http://www.ijbs.com/v10p0136.htm