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Int J Biol Sci 2016; 12(10):1273-1278. doi:10.7150/ijbs.16162

Short Research Communication

An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell

Fan Lin*, Liang Dong*, Weiming Wang, Yuchen Liu, Weiren Huang, Zhiming Cai

State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518039, PR China.
*These authors have contributed equally to this work.

Abstract

Optogenetic gene expression systems enable spatial-temporal modulation of gene transcription and cell behavior. Although applications in biomedicine are emerging, the utility of optogenetic gene switches remains elusive in cancer research due to the relative low gene activation efficiency. Here, we present an optimized CRISPR-Cas9-based light-inducible gene expression device that controls gene transcription in a dose-dependent manner. To prove the potential utility of this device, P53 was tested as a functional target in the bladder cancer cell models. It was illustrated that the light-induced P53 inhibited proliferation of 5637 and UMUC-3 cell effectively. The “light-on” gene expression system may demonstrate a novel therapeutic strategy for bladder cancer intervention.

Keywords: light-inducible gene expression, CRISPR-Cas9, gene switch, bladder cancer.

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How to cite this article:
Lin F, Dong L, Wang W, Liu Y, Huang W, Cai Z. An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. Int J Biol Sci 2016; 12(10):1273-1278. doi:10.7150/ijbs.16162. Available from http://www.ijbs.com/v12p1273.htm