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Int J Biol Sci 2016; 12(10):1273-1278. doi:10.7150/ijbs.16162 This issue Cite

Short Research Communication

An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell

Fan Lin*, Liang Dong*, Weiming Wang, Yuchen Liu, Weiren Huang✉, Zhiming Cai✉

State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518039, PR China.
*These authors have contributed equally to this work.

✉ Corresponding authors: Dr. Weiren Huang Email: pony8980com Or Prof. Zhiming Cai Email: caizhiming2000com.

Citation:
Lin F, Dong L, Wang W, Liu Y, Huang W, Cai Z. An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. Int J Biol Sci 2016; 12(10):1273-1278. doi:10.7150/ijbs.16162. https://www.ijbs.com/v12p1273.htm
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Abstract

Graphic abstract

Optogenetic gene expression systems enable spatial-temporal modulation of gene transcription and cell behavior. Although applications in biomedicine are emerging, the utility of optogenetic gene switches remains elusive in cancer research due to the relative low gene activation efficiency. Here, we present an optimized CRISPR-Cas9-based light-inducible gene expression device that controls gene transcription in a dose-dependent manner. To prove the potential utility of this device, P53 was tested as a functional target in the bladder cancer cell models. It was illustrated that the light-induced P53 inhibited proliferation of 5637 and UMUC-3 cell effectively. The “light-on” gene expression system may demonstrate a novel therapeutic strategy for bladder cancer intervention.

Keywords: light-inducible gene expression, CRISPR-Cas9, gene switch, bladder cancer.

Citation styles

APA
Lin, F., Dong, L., Wang, W., Liu, Y., Huang, W., Cai, Z. (2016). An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. International Journal of Biological Sciences, 12(10), 1273-1278. https://doi.org/10.7150/ijbs.16162.

ACS
Lin, F.; Dong, L.; Wang, W.; Liu, Y.; Huang, W.; Cai, Z. An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. Int. J. Biol. Sci. 2016, 12 (10), 1273-1278. DOI: 10.7150/ijbs.16162.

NLM
Lin F, Dong L, Wang W, Liu Y, Huang W, Cai Z. An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. Int J Biol Sci 2016; 12(10):1273-1278. doi:10.7150/ijbs.16162. https://www.ijbs.com/v12p1273.htm

CSE
Lin F, Dong L, Wang W, Liu Y, Huang W, Cai Z. 2016. An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell. Int J Biol Sci. 12(10):1273-1278.

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