International Journal of Biological Sciences
Impact Factor

ISSN 1449-2288

News feeds of IJBS published articles

Manuscript Status/Login
Contact

Journal of Biomedicinenew

Theranostics

International Journal of Medical Sciences

Journal of Cancer

Oncomedicine

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

Journal of Genomics now in PubMed/PubMed Central. Submit manuscript...

PubMed Central Indexed in Journal Impact Factor

Int J Biol Sci 2017; 13(3):319-326. doi:10.7150/ijbs.17920

Research Paper

Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts

Yongqing Hu1*, Chunmin Zhang1*, Shengli Li2, Ya Jiao3, Tonggang Qi4, Guo Wei1, Gangwen Han1,5✉

1. Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
2. Department of Hematology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
3. Department of Plastic Surgery, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
4. Central Research Laboratory, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
5. Department of Dermatology, Peking University International Hospital, Beijing 102206, China.
* These authors contributed equally to this work.

Abstract

Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB) in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca2+ and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid.

Keywords: keloid fibroblasts, Hypocrellin B, photodynamic therapy, apoptosis

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Hu Y, Zhang C, Li S, Jiao Y, Qi T, Wei G, Han G. Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts. Int J Biol Sci 2017; 13(3):319-326. doi:10.7150/ijbs.17920. Available from http://www.ijbs.com/v13p0319.htm