Int J Biol Sci 2017; 13(3):319-326. doi:10.7150/ijbs.17920
Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
1. Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB) in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca2+ and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid.
Keywords: keloid fibroblasts, Hypocrellin B, photodynamic therapy, apoptosis
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How to cite this article:
Hu Y, Zhang C, Li S, Jiao Y, Qi T, Wei G, Han G. Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts. Int J Biol Sci 2017; 13(3):319-326. doi:10.7150/ijbs.17920. Available from http://www.ijbs.com/v13p0319.htm