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Int J Biol Sci 2017; 13(5):660-668. doi:10.7150/ijbs.19194

Research Paper

Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway

Jianwei Dai1*, Yuyin Lin1*, Youfa Duan2*, Zixuan Li2, Dalei Zhou2, Wensheng Chen1, Lijing Wang2✉, Qian-Qian Zhang2✉

1. GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 510000, China;
2. Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China.
* These authors contributed equally to this work.


Angiogenesis provides nutrients and oxygen to promote tumor growth and affords a channel that facilitates tumor cell entry into the circulation. Andrographolide (Andro) possess anti-tumor activity; however, its direct effect on angiogenesis still needs to be clarified. In this study, our experiments revealed that Andro significantly inhibited vascular growth in chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models. Meanwhile, tumor angiogenesis was also suppressed by Andro. Additionally, we found that cell proliferation, migration and tube formation of vascular endothelial cells was inhibited by Andro treatment in vitro. The effect was primarily mediated through inhibition of miR-21-5p expression and further targeting of TIMP3. This work provides evidence that Andro directly inhibits angiogenesis and might be an effective anti-angiogenic therapeutic drug for cancer treatment.

Keywords: Andrographolide, angiogenesis, miR-21-5p, TIMP3.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( See for full terms and conditions.
How to cite this article:
Dai J, Lin Y, Duan Y, Li Z, Zhou D, Chen W, Wang L, Zhang QQ. Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. Int J Biol Sci 2017; 13(5):660-668. doi:10.7150/ijbs.19194. Available from