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Int J Biol Sci 2018; 14(2):228-236. doi:10.7150/ijbs.22849

Research Paper

MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3

Chengling Chu1, Xin Liu2, Xue Bai2, Tong Zhao2, Mengxue Wang2, Ranchen Xu2, Mingqi Li2, Yingying Hu3, Weihua Li3, Lida Yang2, Youyou Qin4, Meng Yang1, Chaoqi Yan4✉, Yong Zhang2,5✉

1. Department of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;
2. Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150081, China;
3. Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;
4. Department of Breast Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;
5. Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin 150086, China

Abstract

Breast cancer (BC) is the most common cause of death in women throughout the world. Although microRNAs (miRNAs) have been identified as novel regulators in carcinogenesis, there are still abundant hidden treasure needed to be excavated. In the present study, we found that miR-519d expression was remarkably decreased in both human BC tissues and MCF-7 cells. CCK8 and 5-Ethynyl-2′-deoxyuridine (EdU) assays were used to evaluate cell proliferation. Wound-healing and transwell assays were performed for detection of cell migration and invasion. The results demonstrated miR-519d overexpression dramatically suppressed MCF-7 cells proliferation, migration and invasion. While downregulation of miR-519d by miR-519d inhibitor substantially increased MCF-7 cell carcinogenesis. Further analysis identified Matrix Metalloproteinase-3 (MMP3) as a direct target of miR-519d. QRT-PCR and western blot results indicated the correlative expression of miR-519d and MMP3 in BC tissues and MCF-7 cells. In summary, our data uncovered the novel molecular interaction between miR-519d and MMP3, indicating a therapeutic strategy of miR-519d for BC.

Keywords: breast cancer, metastasis, miR-519d, MMP3

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Chu C, Liu X, Bai X, Zhao T, Wang M, Xu R, Li M, Hu Y, Li W, Yang L, Qin Y, Yang M, Yan C, Zhang Y. MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3. Int J Biol Sci 2018; 14(2):228-236. doi:10.7150/ijbs.22849. Available from http://www.ijbs.com/v14p0228.htm