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Int J Biol Sci 2019; 15(3):657-667. doi:10.7150/ijbs.30099 This issue Cite

Research Paper

EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1

Kyoungmi Min, Suk Kyeong Lee✉

Department of Biomedicine & Health Sciences, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea

✉ Corresponding author: Suk Kyeong Lee, Mailing address: Department of Biomedicine & Health Sciences, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of K More

Citation:
Min K, Lee SK. EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. Int J Biol Sci 2019; 15(3):657-667. doi:10.7150/ijbs.30099. https://www.ijbs.com/v15p0657.htm
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Abstract

Graphic abstract

In Epstein-Barr virus (EBV)-infected epithelial cancers, BamHI A rightward transcript (BART) miRNAs are highly expressed. However, only a few target genes of BART miRNAs have been investigated. Our mRNA microarray data showed that DKK1 was markedly down-regulated in EBV-associated gastric carcinoma (EBVaGC) cells. Using luciferase reporter assay we tested whether miR-BART10-3p regulates DKK1 by directly targeting the 3'-UTR of DKK1 mRNA. We observed that miR-BART10-3p transfection decreased DKK1 expression, while an LNA inhibitor of miR-BART10-3p (LNA-miR-BART10-3p(i)) increased DKK1 expression. Furthermore, miR-BART10-3p and siDKK1 promoted cell proliferation and migration. In contrast, transfecting GC cells with LNA-miR-BART10-3p(i) or DKK1 over expression vector suppressed cell proliferation and migration. Our results suggest that miR-BART10-3p may be involved in the tumor progression of EBVaGC by targeting DKK1.

Keywords: Epstein-Barr virus, BART miRNA, DKK1, cell proliferation, cell migration

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APA
Min, K., Lee, S.K. (2019). EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. International Journal of Biological Sciences, 15(3), 657-667. https://doi.org/10.7150/ijbs.30099.

ACS
Min, K.; Lee, S.K. EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. Int. J. Biol. Sci. 2019, 15 (3), 657-667. DOI: 10.7150/ijbs.30099.

NLM
Min K, Lee SK. EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. Int J Biol Sci 2019; 15(3):657-667. doi:10.7150/ijbs.30099. https://www.ijbs.com/v15p0657.htm

CSE
Min K, Lee SK. 2019. EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. Int J Biol Sci. 15(3):657-667.

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