POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells

Huang, Min; Liao, Xiaohua; Wang, Xuepeng; Qian, Yiwei; Zhang, Wensheng; Chen, Guokai; Wu, Qiang

doi:10.7150/ijbs.83927

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Int J Biol Sci 2024; 20(4):1142-1159. doi:10.7150/ijbs.83927 This issue Cite

Research Paper

POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells

Min Huang¹, Xiaohua Liao¹, Xuepeng Wang¹, Yiwei Qian¹, Wensheng Zhang², Guokai Chen³, Qiang Wu^1,4✉

1. The State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao SAR 999078, China.
2. Cam-Su Genomic Resource Center, Medical College of Soochow University, Suzhou 215123, China.
3. Faculty of Health Sciences, University of Macau, Taipa, Macao SAR 999078, China.
4. The Precision Regenerative Medicine Research Centre, Macau University of Science and Technology, Taipa, Macao SAR 999078, China.

Citation:

Huang M, Liao X, Wang X, Qian Y, Zhang W, Chen G, Wu Q. POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells. Int J Biol Sci 2024; 20(4):1142-1159. doi:10.7150/ijbs.83927. https://www.ijbs.com/v20p1142.htm

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Abstract

Human embryonic stem cells (hESCs) can proliferate infinitely (self-renewal) and give rise to almost all types of somatic cells (pluripotency). Hence, understanding the molecular mechanism of pluripotency regulation is important for applications of hESCs in regenerative medicine. Here we report that PATZ1 is a key factor that regulates pluripotency and metabolism in hESCs. We found that depletion of PATZ1 is associated with rapid downregulation of master pluripotency genes and prominent deceleration of cell growth. We also revealed that PATZ1 regulates hESC pluripotency though binding the regulatory regions of OCT4 and NANOG. In addition, we demonstrated PATZ1 is a key node in the OCT4/NANOG transcriptional network. We further revealed that PATZ1 is essential for cell growth in hESCs. Importantly, we discovered that depletion of PATZ1 drives hESCs to exploit glycolysis which energetically compensates for the mitochondrial dysfunction. Overall, our study establishes the fundamental role of PATZ1 in regulating pluripotency in hESCs. Moreover, PATZ1 is essential for maintaining a steady metabolic homeostasis to refine the stemness of hESCs.

Keywords: PATZ1, human embryonic stem cells (hESCs), pluripotency, mitochondrial function, glycolysis

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APA

Huang, M., Liao, X., Wang, X., Qian, Y., Zhang, W., Chen, G., Wu, Q. (2024). POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells. International Journal of Biological Sciences, 20(4), 1142-1159. https://doi.org/10.7150/ijbs.83927.

ACS

Huang, M.; Liao, X.; Wang, X.; Qian, Y.; Zhang, W.; Chen, G.; Wu, Q. POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells. Int. J. Biol. Sci. 2024, 20 (4), 1142-1159. DOI: 10.7150/ijbs.83927.

NLM

CSE

Huang M, Liao X, Wang X, Qian Y, Zhang W, Chen G, Wu Q. 2024. POZ/BTB and AT hook containing zinc finger 1 (PATZ1) suppresses differentiation and regulates metabolism in human embryonic stem cells. Int J Biol Sci. 20(4):1142-1159.

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