Int J Biol Sci 2008; 4(1):29-36. doi:10.7150/ijbs.4.29
Reduced white fat mass in adult mice bearing a truncated Patched 1
Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development. In this report, we found that Patched 1 (Ptc1), a negative regulator of Hh signaling, was expressed in the epididymal fat pad of adult mice. Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1mes/mes), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region. Increased expression of truncated Ptc1, Ptc2 and Gli1, the indicators of ectopic activation of Hh signaling, was observed in epididymal fat pads of adult Ptc1mes/mes mice. In contrast, expression of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha, adipocyte P2 and adipsin were reduced in epididymal fat pads of adult Ptc1mes/mes mice. Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.
Keywords: gene expression, mouse, Patched 1, spontaneous mesenchymal dysplasia, white fat mass, body weight
Li Z, Zhang H, Denhard LA, Liu LH, Zhou H, Lan ZJ. Reduced white fat mass in adult mice bearing a truncated Patched 1. Int J Biol Sci 2008; 4(1):29-36. doi:10.7150/ijbs.4.29. Available from http://www.ijbs.com/v04p0029.htm