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Int J Biol Sci 2010; 6(4):350-360. doi:10.7150/ijbs.6.350

Research Paper

Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs

Yang Li1, Zaiyan Xu1, Hongying Li2, Yuanzhu Xiong1, Bo Zuo1

1. Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
2. Bioengineering Institute, Shanxi Agricultural University, 030801, P. R. China

Abstract

In order to better understand and elucidate the major determinants of red and white muscle phenotypic properties, the global gene expression profiling was performed in white (longissimus doris) and red (soleus) skeletal muscle of Chinese Meishan pigs using the Affymetrix Porcine Genechip. 550 transcripts at least 1.5-fold difference were identified at p < 0.05 level, with 323 showing increased expression and 227 decreased expression in red muscle. Quantitative real-time PCR validated the differential expression of eleven genes (α-Actin, ART3, GATA-6, HMOX1, HSP, MYBPH, OCA2, SLC12A4, TGFB1, TGFB3 and TNX). Twenty eight signaling pathways including ECM-receptor interaction, focal adhesion, TGF-beta signaling pathway, MAPK signaling pathway, Wnt signaling pathway, mTOR signaling pathway, insulin signaling pathway and cell cycle, were identified using KEGG pathway database. Our findings demonstrate previously unrecognized changes in gene transcription between red and white muscle, and some potential cascades identified in the study merit further investigation.

Keywords: Affymetrix, Differential transcriptional analysis, Longissimus doris, Pig, Soleus

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How to cite this article:
Li Y, Xu Z, Li H, Xiong Y, Zuo B. Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs. Int J Biol Sci 2010; 6(4):350-360. doi:10.7150/ijbs.6.350. Available from http://www.ijbs.com/v06p0350.htm