International Journal of Biological Sciences

Impact factor
3.873

ISSN 1449-2288

News feeds of IJBS published articles
My Manuscript
My Account

Journal of Biomedicinenew

Theranostics

International Journal of Medical Sciences

Journal of Cancer

Oncomedicine

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

PubMed Central Indexed in Journal Impact Factor

Int J Biol Sci 2012; 8(1):66-78. doi:10.7150/ijbs.8.66

Research Paper

Proteomic Analysis on Acetate Metabolism in Citrobacter sp. BL-4

Young-Man Kim1*, Sung-Eun Lee2*, Byeoung-Soo Park2, Mi-Kyung Son2, Young-Mi Jung3, Seung-Ok Yang4, Hyung-Kyoon Choi4, Sung-Ho Hur5, Jong Hwa Yum6✉

1. Department of Food Science and Nutrition, Dong-eui University, Busan 614-714, Republic of Korea
2. Research Station, Nanotoxtech Inc., Gyeonggi Technopark Technology Development Center, Ansan 426-901, Republic of Korea
3. Department of Genetic Engineering, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea
4. College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea
5. Department of Clinical Laboratory Science, Dong-eui Institute of Technology University, Busan, 614-715, Korea
6. Department of Clinical Laboratory Science, Dong-eui University, Busan 614-714, Republic of Korea
* The same contribution to the paper.

Abstract

Mass production of glucosamine (GlcN) using microbial cells is a worthy approach to increase added values and keep safety problems in GlcN production process. Prior to set up a microbial cellular platform, this study was to assess acetate metabolism in Citrobacter sp. BL-4 (BL-4) which has produced a polyglucosamine PGB-2. The LC-MS analysis was conducted after protein separation on the 1D-PAGE to accomplish the purpose of this study. 280 proteins were totally identified and 188 proteins were separated as acetate-related proteins in BL-4. Acetate was converted to acetyl-CoA by acetyl-CoA synthetase up-regulated in the acetate medium. The glyoxylate bypass in the acetate medium was up-regulated with over-expression of isocitrate lyases and 2D-PAGE confirmed this differential expression. Using 1H-NMR analysis, the product of isocitrate lyases, succinate, increased about 15 times in the acetate medium. During acetate metabolism proteins involved in the lipid metabolism and hexosamine biosynthesis were over-expressed in the acetate medium, while proteins involved in TCA cycle, pentose phosphate cycle and purine metabolism were down-regulated. Taken together, the results from the proteomic analysis can be applied to improve GlcN production and to develop metabolic engineering in BL-4.

Keywords: Citrobacter sp. BL-4, proteomics, 1H-NMR, acetate metabolism, polyglucosamine production

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Kim YM, Lee SE, Park BS, Son MK, Jung YM, Yang SO, Choi HK, Hur SH, Yum JH. Proteomic Analysis on Acetate Metabolism in Citrobacter sp. BL-4. Int J Biol Sci 2012; 8(1):66-78. doi:10.7150/ijbs.8.66. Available from http://www.ijbs.com/v08p0066.htm