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Int J Biol Sci 2015; 11(6):664-671. doi:10.7150/ijbs.10783 This issue Cite

Research Paper

A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway

Lihua Sun, Chao Xu, Guoqing Chen, Min Yu, Songwei Yang, Yuan Qiu, Ke Peng, Wensheng Wang, Weidong Xiao✉, Hua Yang✉

Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

✉ Corresponding authors: Hua Yang, M.D., Ph.D. or Weidong Xiao, M.D., Ph.D.. Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China, Phone: (0086)23-687-55705 (Office), E-mail: huayangedu.cn o More

Citation:
Sun L, Xu C, Chen G, Yu M, Yang S, Qiu Y, Peng K, Wang W, Xiao W, Yang H. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. Int J Biol Sci 2015; 11(6):664-671. doi:10.7150/ijbs.10783. https://www.ijbs.com/v11p0664.htm
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Abstract

Graphic abstract

OS-9 is a lectin required for efficient ubquitination of glycosylated substrates of endoplasmic reticulum-associated degradation (ERAD). OS-9 has previously been implicated in ER-to-Golgi transport and transcription factor turnover. However, we know very little about other functions of OS-9 under endoplasmic reticulum stress. Here, we used gene knockdown and overexpression approaches to study the protective effect of OS-9 on intestinal barrier function of intestinal epithelial cell Caco-2 monolayer. We found that OS-9 attenuated intestinal epithelial barrier dysfunction under hypoxia through up-regulating occludin and claudin-1 protein expression. Furthermore, we showed that the up-regulation of occludin and claudin-1 induced by OS-9 was mediated by p38 and ERK1/2 phosphorylation and did not involve HIF-1α. In summary, our results demonstrate that OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition.

Keywords: OS-9, intestinal epithelial barrier, p38, tight junction

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APA
Sun, L., Xu, C., Chen, G., Yu, M., Yang, S., Qiu, Y., Peng, K., Wang, W., Xiao, W., Yang, H. (2015). A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. International Journal of Biological Sciences, 11(6), 664-671. https://doi.org/10.7150/ijbs.10783.

ACS
Sun, L.; Xu, C.; Chen, G.; Yu, M.; Yang, S.; Qiu, Y.; Peng, K.; Wang, W.; Xiao, W.; Yang, H. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. Int. J. Biol. Sci. 2015, 11 (6), 664-671. DOI: 10.7150/ijbs.10783.

NLM
Sun L, Xu C, Chen G, Yu M, Yang S, Qiu Y, Peng K, Wang W, Xiao W, Yang H. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. Int J Biol Sci 2015; 11(6):664-671. doi:10.7150/ijbs.10783. https://www.ijbs.com/v11p0664.htm

CSE
Sun L, Xu C, Chen G, Yu M, Yang S, Qiu Y, Peng K, Wang W, Xiao W, Yang H. 2015. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. Int J Biol Sci. 11(6):664-671.

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