Int J Biol Sci 2015; 11(8):913-922. doi:10.7150/ijbs.11585 This issue Cite

Research Paper

Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7

Xiangjun Song*, Xiaomin Zhao*, Yong Huang, Hailing Xiang, Wenlong Zhang, Dewen Tong

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China
* These authors contributed equally to this work.

Citation:
Song X, Zhao X, Huang Y, Xiang H, Zhang W, Tong D. Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. Int J Biol Sci 2015; 11(8):913-922. doi:10.7150/ijbs.11585. https://www.ijbs.com/v11p0913.htm
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Abstract

Graphic abstract

Transmissible gastroenteritis virus (TGEV) is a member of Coronaviridae family. TGEV infection has emerged as a major cause of severe gastroenteritis and leads to alterations of many cellular processes. Meanwhile, the pathogenic mechanism of TGEV is still unclear. microRNAs (miRNAs) are a novel class of small non-coding RNAs which are involved in the regulation of numerous biological processes such as viral infection and cell apoptosis. Accumulating data show that miRNAs are involved in the process of coronavirus infection such as replication of severe acute respiratory syndrome coronavirus (SARS-CoV). However, the link between miRNAs and TGEV infection is unknown. In this study, we performed microRNA microarray assay and predicted targets of altered miRNAs. The results showed TGEV infection caused the change of miRNAs profile. Then we selected miR-4331 for further analysis and subsequently identified cell division cycle-associated protein 7 (CDCA7) as the target of miR-4331. Moreover, miR-4331 showed the ability to inhibit transcription of TGEV gene 7 (a non-structure gene) via directly targeting CDCA7. In conclusion, differentially expressed miR-4331 that is caused by TGEV infection can suppress transcription of TGEV gene 7 via targeting cellular CDCA7. Our key finding is that TGEV selectively manipulates the expression of some cellular miRNAs to regulate its subgenomic transcription.

Keywords: TGEV, microRNAs (miRNAs), TGEV, CDCA7, Gene 7, viral transcription


Citation styles

APA
Song, X., Zhao, X., Huang, Y., Xiang, H., Zhang, W., Tong, D. (2015). Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. International Journal of Biological Sciences, 11(8), 913-922. https://doi.org/10.7150/ijbs.11585.

ACS
Song, X.; Zhao, X.; Huang, Y.; Xiang, H.; Zhang, W.; Tong, D. Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. Int. J. Biol. Sci. 2015, 11 (8), 913-922. DOI: 10.7150/ijbs.11585.

NLM
Song X, Zhao X, Huang Y, Xiang H, Zhang W, Tong D. Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. Int J Biol Sci 2015; 11(8):913-922. doi:10.7150/ijbs.11585. https://www.ijbs.com/v11p0913.htm

CSE
Song X, Zhao X, Huang Y, Xiang H, Zhang W, Tong D. 2015. Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. Int J Biol Sci. 11(8):913-922.

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