Int J Biol Sci 2019; 15(10):2211-2223. doi:10.7150/ijbs.35266

Research Paper

Tanshinone IIA attenuates demyelination and promotes remyelination in A. cantonensis-infected BALB/c mice

Ying Feng1, Feng Feng4, Cunjing Zheng5, Zongpu Zhou1, Meihua Jiang6, Zhen Liu7, Fukang Xie5, Xi Sun2,3✉, Zhongdao Wu2,3✉

1. Medical School of South China University of Technology, Guangzhou, China
2. Parasitology Department of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
3. Key Laboratory of Tropical Disease Control (SYSU), Ministry of Education, Guangzhou, China
4. The Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China
5. Histology and Embryology Department of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
6. Anatomy Department of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
7. Guangzhou First People's Hospital, Guangzhou, China

Abstract

Background: Angiostrongylus cantonensis infection can cause demyelination in the central nervous system, and there is no effective treatment. Methods: We used dexamethasone, Tanshinone IIA (TSIIA) and Cryptotanshinone(Two traditional Chinese medicine monomers) in combination with albendazole (AB, a standard anti-helminthic compound) to observe their therapeutic effect on demyelination in A. cantonensis-infected mice. Luxol fast blue staining and electron microscope of myelin sheath, Oligodendrocyte (OL) number and myelin basic protein (MBP) expression in brain was detected in above groups. Results: TSIIA+AB facilitated OL proliferation and significantly increased both myelin sheath thickness and the population of small-diameter axons. In addition, TSIIA treatment inhibited the expression of inflammation-related factors (interleukin [IL]-6, IL-1β, tumor necrosis factor [TNF]-α, inducible nitric oxide synthase [iNOS]) rather than inhibiting eosinophil infiltration in brain. TSIIA also decreased microglial activation and shifted their phenotype from M1 to M2. Conclusions: Taken together, these results provide evidence that TSIIA combined with AB may be an effective treatment for demyelination caused by A. cantonensis infection and other demyelinating diseases.

Keywords: Tanshinone IIA, demyelination, remyelination, microglia polarization, A. cantonensis

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How to cite this article:
Feng Y, Feng F, Zheng C, Zhou Z, Jiang M, Liu Z, Xie F, Sun X, Wu Z. Tanshinone IIA attenuates demyelination and promotes remyelination in A. cantonensis-infected BALB/c mice. Int J Biol Sci 2019; 15(10):2211-2223. doi:10.7150/ijbs.35266. Available from http://www.ijbs.com/v15p2211.htm