Int J Biol Sci
2023; 19(12):3781-3803.
doi:10.7150/ijbs.85063 This issueCite
Research Paper
Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner
Fei Long1,2†, Liang Li1†, Canbin Xie1, Min Ma1, Zhiwei Wu1, Zhixing Lu3, Baiying Liu1, Ming Yang1, Fan Zhang1, Zhengping Ning1, Chonglei Zhong1, Bowen Yu1, Shiyi Liu1,4, Longyu Wan1,4, Buning Tian1, Kaiyan Yang1, Yihang Guo1, Miao Chen1, Jin Chou1, Xiaorong Li1, Gui Hu1, Changwei Lin1✉, Yi Zhang1✉
1. Department of Gastrointestinal Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China. 2. Postdoctoral Research Station of Basic Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China. 3. Department of Gastrointestinal, Hernia and Enterofistula Surgery, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530000, China. 4. School of Basic Medical Science, Central South University, Changsha, Hunan 410078, China. † These authors contributed equally.
Long F, Li L, Xie C, Ma M, Wu Z, Lu Z, Liu B, Yang M, Zhang F, Ning Z, Zhong C, Yu B, Liu S, Wan L, Tian B, Yang K, Guo Y, Chen M, Chou J, Li X, Hu G, Lin C, Zhang Y. Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. Int J Biol Sci 2023; 19(12):3781-3803. doi:10.7150/ijbs.85063. https://www.ijbs.com/v19p3781.htm
Circular RNAs (circRNAs) are covalently closed RNA structures that play multiple roles in tumorigenesis and progression. Compared with exon‒intron circRNAs, the biological functions and implications of intergenic circRNAs in human cancer are still poorly understood. Here, we performed circRNA microarray analysis and identified an intergenic circRNA, circ_0007379, that was significantly downregulated in patients with colorectal cancer (CRC). The biogenesis of circ_0007379 was mediated by reverse complementary matches (RCMs) and was negatively regulated by the RNA helicase DHX9. Functionally, circ_0007379 suppressed CRC cell growth and metastasis in cell culture as well as in patient-derived organoid and xenograft models. Mechanistically, circ_0007379 acted as a scaffold to facilitate the processing of both pri-miR-320a and pre-miR-320a in a KSRP-dependent manner, leading to miR-320a maturation and subsequent repression of transcription factor RUNX1 expression. Thus, our findings establish a previously unrecognized function of circRNA in inhibiting CRC progression.
Long, F., Li, L., Xie, C., Ma, M., Wu, Z., Lu, Z., Liu, B., Yang, M., Zhang, F., Ning, Z., Zhong, C., Yu, B., Liu, S., Wan, L., Tian, B., Yang, K., Guo, Y., Chen, M., Chou, J., Li, X., Hu, G., Lin, C., Zhang, Y. (2023). Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. International Journal of Biological Sciences, 19(12), 3781-3803. https://doi.org/10.7150/ijbs.85063.
ACS
Long, F.; Li, L.; Xie, C.; Ma, M.; Wu, Z.; Lu, Z.; Liu, B.; Yang, M.; Zhang, F.; Ning, Z.; Zhong, C.; Yu, B.; Liu, S.; Wan, L.; Tian, B.; Yang, K.; Guo, Y.; Chen, M.; Chou, J.; Li, X.; Hu, G.; Lin, C.; Zhang, Y. Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. Int. J. Biol. Sci. 2023, 19 (12), 3781-3803. DOI: 10.7150/ijbs.85063.
NLM
Long F, Li L, Xie C, Ma M, Wu Z, Lu Z, Liu B, Yang M, Zhang F, Ning Z, Zhong C, Yu B, Liu S, Wan L, Tian B, Yang K, Guo Y, Chen M, Chou J, Li X, Hu G, Lin C, Zhang Y. Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. Int J Biol Sci 2023; 19(12):3781-3803. doi:10.7150/ijbs.85063. https://www.ijbs.com/v19p3781.htm
CSE
Long F, Li L, Xie C, Ma M, Wu Z, Lu Z, Liu B, Yang M, Zhang F, Ning Z, Zhong C, Yu B, Liu S, Wan L, Tian B, Yang K, Guo Y, Chen M, Chou J, Li X, Hu G, Lin C, Zhang Y. 2023. Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. Int J Biol Sci. 19(12):3781-3803.
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