Int J Biol Sci 2008; 4(5):279-282. doi:10.7150/ijbs.4.279

Short Research Communication

Discovery of four natural clones in a crayfish species Procambarus clarkii

G. H. Yue1✉, G. L. Wang2, B. Q. Zhu3, C. M. Wang1, Z .Y. Zhu1, L. C. Lo1

1. Molecular population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore
2. College of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China
3. College of Animal Sciences, Zhejiang University, Hangzhou, China

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Yue GH, Wang GL, Zhu BQ, Wang CM, Zhu ZY, Lo LC. Discovery of four natural clones in a crayfish species Procambarus clarkii. Int J Biol Sci 2008; 4(5):279-282. doi:10.7150/ijbs.4.279. Available from

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Self-cloning is quite rare in shrimp, lobsters, crayfish and crabs. Here we report the discovery of four natural clones of red swamp crayfish (Procambarus clarkii), each containing 2-6 genetically identical individuals, during the genotyping of 120 individuals with five microsatellites. The four clones were heterozygote at most of the five microsatellite loci. Phylogenetic analysis using microsatellite genotypes suggests recent origin of the four clones. Sequencing a part of the mitochondrial gene Cox I confirmed that the four clones were from the species Procambarus clarkii.

Keywords: crayfish, self-cloning, invasion, microsatellite