Full Text | PDF

Int J Biol Sci 2010; 6(4):327-332. doi:10.7150/ijbs.6.327 This issue Cite

Research Paper

Generation of Fgfr3 Conditional Knockout Mice

Nan Su^1*, Xiaoling Xu^2*, Cuiling Li², Qifen He¹, Ling Zhao¹, Can Li¹, Siyu Chen¹, Fengtao Luo¹, Lingxian Yi¹, Xiaolan Du¹, Haiyang Huang¹, Chuxia Deng^{2 ✉}, Lin Chen^{1 ✉}

1. State Key Laboratory of Trauma, Burns and Combined Injury, Center of Bone Metabolism and Repair, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
2. Genetics of Development and Disease Branch, National Institute of Diabetes, Digestive and Kidney Diseases, US National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892, USA
^*These authors contributed equally to this work

✉ Corresponding author: Lin Chen, State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China. Tel: 86-23-68702991; Fax: 86-23-68702991; E-mail: linchen70com. Chuxia Deng, Genetics of Development and Disease Branch, National Institute of Diabetes, Digestive and Kidney Diseases, US National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892, USA. E-mail:chuxiadniddk.nih.gov More

Abstract

Fibroblast growth factor receptor 3 (FGFR3), highly conserved in both humans and murine, is one of key tyrosine kinase receptors for FGF. FGFR3 is expressed in different tissues, including cartilage, brain, kidney, and intestine at different development stages. Conventional knockout of Fgfr3 alleles leads to short life span, and overgrowth of bone. In clinic, human FGFR3 mutations are responsible for three different types of chondrodysplasia syndromes including achondroplasia (ACH), hypochondroplasia (HCH) and thanatophoric dysplasia (TD). For better understanding of the roles of FGFR3 in different tissues at different stages of development and in pathological conditions, we generated Fgfr3 conditional knockout mice in which loxp sites flank exons 9-10 in the Fgfr3 allele. We also demonstrated that Cre-mediated recombination using Col2a1-Cre, a Cre line expressed in chondrocyte during bone development, results in specific deletion of the gene in tissues containing cartilage. This animal model will be useful to study distinct roles of FGFR3 in different tissues at different ages.

Keywords: FGFR3, conditional knock out, Cre-Loxp, gene targeting

Citation styles

©2024 Ivyspring International Publisher. Terms of use