Int J Biol Sci
2011; 7(3):347-363.
doi:10.7150/ijbs.7.347 This issueCite
Research Paper
MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3
Cong Liu1#, Bailong Li1#, Ying Cheng1#, Jing Lin1, Jun Hao2, Shuyu Zhang3, R.E.J. Mitchel4, Ding Sun1, Jin Ni1, Luqian Zhao1, Fu Gao1✉, Jianming Cai1✉
1. Department of Radiation Medicine, Second Military Medical University, Xiangyin Road, Shanghai 200433, PR China; 2. Department of Pancreatic Surgery, Changhai Hospital, Second Military Medical University, Xiangyin Road, Shanghai 200433, PR China; 3. School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China; 4. Radiation Protection Research and Instrumentation Branch, Atomic Energy of Canada Limited, Chalk River Laboratories, Chalk River, ON, K0J 1J0, Canada. # These authors contributed equally to this work.
✉ Corresponding author: Jianming Cai and Fu Gao. Address: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University; 800, Xiangyin Road 200433, Shanghai; P.R. China. Fax: +86-21-81871150. E-mail: caijianming882003com and gaofu_2002com.cn.More
Citation:
Liu C, Li B, Cheng Y, Lin J, Hao J, Zhang S, Mitchel REJ, Sun D, Ni J, Zhao L, Gao F, Cai J. MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. Int J Biol Sci 2011; 7(3):347-363. doi:10.7150/ijbs.7.347. https://www.ijbs.com/v07p0347.htm
Dysregulation of certain microRNAs (miRNAs) in cancer can promote tumorigenesis, metastasis and invasion. However, the functions and targets of only a few mammalian miRNAs are known. In particular, the miRNAs that participates in radiation induced carcinogenesis and the miRNAs that target the tumor suppressor gene Big-h3 remain undefined. Here in this study, using a radiation induced thymic lymphoma model in BALB/c mice, we found that the tumor suppressor gene Big-h3 is down-regulated and miR-21 is up-regulated in radiation induced thymic lymphoma tissue samples. We also found inverse correlations between Big-h3 protein and miR-21 expression level among different tissue samples. Furthermore, our data indicated that miR-21 could directly target Big-h3 in a 3′UTR dependent manner. Finally, we found that miR-21 could be induced by TGFβ, and miR-21 has both positive and negative effects in regulating TGFβ signaling. We conclude that miR-21 participates in radiation induced carcinogenesis and it regulates TGFβ signaling.
Liu, C., Li, B., Cheng, Y., Lin, J., Hao, J., Zhang, S., Mitchel, R.E.J., Sun, D., Ni, J., Zhao, L., Gao, F., Cai, J. (2011). MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. International Journal of Biological Sciences, 7(3), 347-363. https://doi.org/10.7150/ijbs.7.347.
ACS
Liu, C.; Li, B.; Cheng, Y.; Lin, J.; Hao, J.; Zhang, S.; Mitchel, R.E.J.; Sun, D.; Ni, J.; Zhao, L.; Gao, F.; Cai, J. MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. Int. J. Biol. Sci. 2011, 7 (3), 347-363. DOI: 10.7150/ijbs.7.347.
NLM
Liu C, Li B, Cheng Y, Lin J, Hao J, Zhang S, Mitchel REJ, Sun D, Ni J, Zhao L, Gao F, Cai J. MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. Int J Biol Sci 2011; 7(3):347-363. doi:10.7150/ijbs.7.347. https://www.ijbs.com/v07p0347.htm
CSE
Liu C, Li B, Cheng Y, Lin J, Hao J, Zhang S, Mitchel REJ, Sun D, Ni J, Zhao L, Gao F, Cai J. 2011. MiR-21 plays an Important Role in Radiation Induced Carcinogenesis in BALB/c Mice by Directly Targeting the Tumor Suppressor Gene Big-h3. Int J Biol Sci. 7(3):347-363.