Int J Biol Sci 2011; 7(5):567-574. doi:10.7150/ijbs.7.567

Short Research Communication

miR-148a Promoted Cell Proliferation by Targeting p27 in Gastric Cancer Cells

Shui-Long Guo1#, Zheng Peng2#, Xue Yang1,3, Kai-Ji Fan1, Hui Ye1,3, Zhen-Hua Li1, Yan Wang4, Xiao-Li Xu5, Jun Li1, You-Liang Wang1, Yan Teng1✉, Xiao Yang1,3 ✉

1. State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, P.R. China
2. General Surgery Department, PLA General Hospital & PLA Postgraduate School of Medicine, Beijing, P.R. China
3. Model Organism Division, E-institutes of Shanghai Universities, Shanghai Jiaotong University, P.R. China
4. General Surgery Department, China-Japan Friendship Hospital, Beijing, P.R. China
5. Neurosurgery Department, China-Japan Friendship Hospital, Beijing, P.R. China
# Shui-Long Guo and Zheng Peng contributed equally to this work.

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Citation:
Guo SL, Peng Z, Yang X, Fan KJ, Ye H, Li ZH, Wang Y, Xu XL, Li J, Wang YL, Teng Y, Yang X. miR-148a Promoted Cell Proliferation by Targeting p27 in Gastric Cancer Cells. Int J Biol Sci 2011; 7(5):567-574. doi:10.7150/ijbs.7.567. Available from http://www.ijbs.com/v07p0567.htm

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Abstract

Accumulating evidence has shown that miRNAs are aberrantly expressed in human gastric cancer and crucial to tumorigenesis. Herein, we identified the role of miR-148a in gastric cell proliferation. miR-148a knockdown inhibited cell proliferation in gastric cancer cell lines. Conversely, miR-148a overexpression promoted cell proliferation and cell cycle progression. p27, a key inhibitor of cell cycle, was verified as the target of miR-148a, indicating miR-148a might downregulate p27 expression to promote gastric cell proliferation. Moreover, we confirmed that miR-148a expression was frequently and dramatically downregulated in human advanced gastric cancer tissues, and observed a good inverse correlation between miR-148a and p27 expression in tumor samples. Thus, our results demonstrated that miR-148a downregulation might exert some sort of antagonistic function in cell proliferation, rather than promote cell proliferation in gastric cancer.

Keywords: gastric cancer, cell proliferation, miR-148a, p27